Saito M, Kondo A, Gotoh M, Kato K, Levin R M
Department of Urology, Nagoya University School of Medicine, Japan.
Neurourol Urodyn. 1993;12(2):191-200. doi: 10.1002/nau.1930120214.
The effect of age on the contractile response of the rat urinary bladder to various neurotransmitters was studied using in vitro detrusor strips isolated from female Wistar rats. The contractile responses to 8 neurotransmitters were determined for rat urinary bladders isolated from 3 age groups: 24, 16, and 6 months old. The results can be summarized as follows: 1) There was no age-dependent change in the maximum response to KCl. 2) The contractile response to norepinephrine, adenosine 5'-triphosphate (ATP), and serotonin increased with age. 3) The relaxation response to isoproterenol was significantly less in 24-month-old rats than in the other 2 age groups. 4) There were no age related changes in the responses to acetylcholine, prostaglandin F2 alpha, and angiotensin II. 5) Vasoactive intestinal polypeptide (VIP) failed to induce any response in the rat bladders of any group. 6) There were no significant differences in the ED50s for the 5 neurotransmitters among the 3 age groups. In summary, significant differences in the response to adrenergic, purinergic, and serotonergic stimulation were found as a function of age, whereas no age related changes in the response to cholinergic or generalized depolarization with KCl were found.
利用从雌性Wistar大鼠分离出的体外逼尿肌条，研究了年龄对大鼠膀胱对各种神经递质收缩反应的影响。测定了从3个年龄组（24、16和6月龄）分离出的大鼠膀胱对8种神经递质的收缩反应。结果总结如下：1）对氯化钾的最大反应不存在年龄依赖性变化。2）对去甲肾上腺素、5'-三磷酸腺苷（ATP）和5-羟色胺的收缩反应随年龄增加。3）24月龄大鼠对异丙肾上腺素的舒张反应明显低于其他2个年龄组。4）对乙酰胆碱、前列腺素F2α和血管紧张素II的反应不存在与年龄相关的变化。5）血管活性肠肽（VIP）未能在任何组的大鼠膀胱中诱导任何反应。6）3个年龄组中5种神经递质的半数有效量（ED50）无显著差异。总之，发现对肾上腺素能、嘌呤能和5-羟色胺能刺激的反应存在与年龄相关的显著差异，而对胆碱能刺激或氯化钾引起的全身性去极化反应未发现与年龄相关的变化。
