Weiss M, Buhl R, Mirow N, Birkhahn A, Schneider M, Wernet P
Department of Anesthesiology, Heinrich-Heine-Universität, Düsseldorf, Germany.
J Crit Care. 1994 Jun;9(2):83-9. doi: 10.1016/0883-9441(94)90018-3.
The dose-response relationship of commercially available preparations of methohexital, pentobarbital, phenobarbital, and thiopental and their respective drug-free solutions on granulocyte function was investigated to evaluate whether suppression of neutrophil chemiluminescence is mediated by the barbiturates themselves or by their drug-free solutions. Furthermore, it was assessed whether suppression of chemiluminescence is due to an interaction mainly with neutrophils or to free radical scavenging.
The dose-response effects of the four barbiturates on granulocyte function were tested by zymosan-induced neutrophil chemiluminescence and, in addition, in a cell-free chemiluminescence system.
Methohexital and pentobarbital did not influence zymosan-induced neutrophil chemiluminescence, whereas phenobarbital and thiopental decreased neutrophil chemiluminescence in a dose-dependent fashion. Nonphysiological osmolality (531 mosmol/kg) caused this impaired neutrophil chemiluminescence at the greatest concentration of phenobarbital. Thiopental solely suppressed neutrophil chemiluminescence drug specifically. Because thiopental also reduced chemiluminescence generated in a cell-free system, free radical scavenging might contribute to the impaired neutrophil chemiluminescence observed with thiopental.
With the exception of thiopental, barbiturates do not impair oxygen radical production during phagocytosis of neutrophils.
研究市售美索比妥、戊巴比妥、苯巴比妥和硫喷妥钠制剂及其各自的无药溶液对粒细胞功能的剂量反应关系，以评估中性粒细胞化学发光的抑制是由巴比妥类药物本身还是其无药溶液介导的。此外，还评估了化学发光的抑制是主要由于与中性粒细胞的相互作用还是自由基清除作用。
通过酵母聚糖诱导的中性粒细胞化学发光以及在无细胞化学发光系统中测试四种巴比妥类药物对粒细胞功能的剂量反应效应。
美索比妥和戊巴比妥不影响酵母聚糖诱导的中性粒细胞化学发光，而苯巴比妥和硫喷妥钠则以剂量依赖性方式降低中性粒细胞化学发光。在苯巴比妥最高浓度时，非生理渗透压（531毫摩尔/千克）导致这种中性粒细胞化学发光受损。硫喷妥钠仅特异性地抑制中性粒细胞化学发光。由于硫喷妥钠也降低了无细胞系统中产生的化学发光，自由基清除可能导致硫喷妥钠观察到的中性粒细胞化学发光受损。
除硫喷妥钠外，巴比妥类药物不损害中性粒细胞吞噬过程中的氧自由基产生。
