Redondo A, Villanueva M J, Rodríguez M D
Departamento de Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
J Chromatogr A. 1994 Aug 19;677(2):273-8. doi: 10.1016/0021-9673(94)80155-X.
An HPLC method was developed to determine dietary fibre in carrots (Daucus carota L.). Primary hydrolysis of dietary fibre residue was performed with 12 M H2SO4 for 2 h at 40 degrees C and secondary hydrolysis with 0.414 M H2SO4 for 3 h at 100 degrees C. For the neutralization step prior to injection, AG4-X4 resin (Bio-Rad) was used. Neutral monosaccharides were separated using an HPX-87P column (Bio-Rad). This method was applied for evaluation of the quantitative variation of dietary fibre content in carrots during autoclaving.
开发了一种高效液相色谱法来测定胡萝卜（胡萝卜属）中的膳食纤维。膳食纤维残渣先用12 M硫酸在40℃下进行2小时的一次水解，然后用0.414 M硫酸在100℃下进行3小时的二次水解。在进样前的中和步骤中，使用了AG4-X4树脂（伯乐公司）。中性单糖通过HPX-87P柱（伯乐公司）进行分离。该方法用于评估高压灭菌过程中胡萝卜膳食纤维含量的定量变化。
