Bergler H, Abraham D, Aschauer H, Turnowsky F
Institut für Mikrobiologie, Karl-Franzens-Universität, Graz, Austria.
Microbiology (Reading). 1994 Aug;140 ( Pt 8):1937-44. doi: 10.1099/13500872-140-8-1937.
Treatment of Escherichia coli with diazaborine strongly induces the synthesis of a 28 kDa protein which is associated with the cytoplasmic membrane. The partial amino acid sequence proved that this protein is identical to the phage shock protein PspA. The kinetics of the expression of the pspA gene were determined in an E. coli strain which carried a pspA-lacZ fusion in the chromosome. PspA synthesis is independent of the growth phase. It is, however, strongly induced when fatty acid biosynthesis is inhibited by diazaborine or cerulenin. Treatment with either compound also causes dose-dependent inhibition of phospholipid biosynthesis whose degree correlates with the induction of PspA. Another cause of induction of PspA synthesis is treatment of E. coli with globomycin, which is an inhibitor of the processing of lipoproteins.
用重氮硼烷处理大肠杆菌会强烈诱导一种与细胞质膜相关的28 kDa蛋白质的合成。部分氨基酸序列证明该蛋白质与噬菌体休克蛋白PspA相同。在染色体中携带pspA - lacZ融合基因的大肠杆菌菌株中测定了pspA基因的表达动力学。PspA的合成与生长阶段无关。然而，当脂肪酸生物合成被重氮硼烷或浅蓝菌素抑制时，它会被强烈诱导。用这两种化合物处理也会导致磷脂生物合成的剂量依赖性抑制，其程度与PspA的诱导相关。诱导PspA合成的另一个原因是用球霉素处理大肠杆菌，球霉素是一种脂蛋白加工抑制剂。
