Michel G P
Laboratoire de Chimie Bactérienne, Marseille, France.
J Bacteriol. 1993 May;175(10):3228-31. doi: 10.1128/jb.175.10.3228-3231.1993.
The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the foreign gene restored a thermoresistant phenotype but failed to modulate the heat shock response in E. coli.
运动发酵单胞菌的DnaK蛋白（DnaKz）被鉴定出来，基于N端21个氨基酸的序列，发现它与大肠杆菌的DnaK蛋白有80%的同源性。dnaKz基因被克隆，并发现它在大肠杆菌的一个温度敏感型dnaK突变体中表达。外源基因的表达恢复了耐热表型，但未能调节大肠杆菌中的热休克反应。
