Rabian-Herzog C, Lesage S, Gluckman E, Charron D
Laboratory of Immunology and Histocompatibility, Hôpital Saint-Louis, Paris, France.
J Hematother. 1993 Summer;2(2):255-7. doi: 10.1089/scd.1.1993.2.255.
Twenty-seven cord blood samples from healthy newborns were processed according to a "whole blood" flow cytometric analysis. The CD3-positive T cells were a variable subpopulation representing 44.8 +/- 13.3% of lymphocytes. The majority of the CD3+ cells are CD38+. Newborn T cells have lower levels of both IL-2 receptors and HLA-DR than do adult T cells. The CD4-positive T cells represented 31.0 +/- 10.8% of lymphocytes with a great prevalence of the CD4+/CD45RA+ population. The CD3+/CD8+/CD11b+ cells are increased to 23.4 +/- 7.1% of lymphocytes. The CD57 antigen is not expressed. The NK population, CD16+/CD56+, is increased to 25 +/- 11% of lymphocytes. Of CD19+ cord blood B lymphocytes 68% coexpressed CD5. Thus "suppressive" and "naive" cells are prominently represented in cord blood.
对来自健康新生儿的27份脐带血样本进行了“全血”流式细胞术分析。CD3阳性T细胞是一个可变的亚群，占淋巴细胞的44.8±13.3%。大多数CD3+细胞是CD38+。新生儿T细胞的IL-2受体和HLA-DR水平均低于成人T细胞。CD4阳性T细胞占淋巴细胞的31.0±10.8%，其中CD4+/CD45RA+群体占比很高。CD3+/CD8+/CD11b+细胞增加至淋巴细胞的23.4±7.1%。CD57抗原未表达。NK群体，即CD16+/CD56+，增加至淋巴细胞的25±11%。在脐带血CD19+B淋巴细胞中，68%共表达CD5。因此，“抑制性”和“幼稚”细胞在脐带血中占显著比例。
