Paget S A, McMaster P R, van Boxel J A
J Immunol. 1976 Dec;117(6):2267-9.
Experimental autoimmune thyroiditis was induced in out-bred guinea pigs by isoimmunization with thyroid extract in complete Freund's adjuvant. A digestion procedure using collagenase and deoxyribonuclease was used to make viable single-cell suspensions of pooled thyroid glands from groups of animals with advanced degrees of thyroiditis. Thymus-derived or T lymphocytes, identified by their capacity to form E rosettes with rabbit erythrocytes, were found to be the predominant (75%) infiltrating lymphocyte; bone marrow-derived or B cells consitituted most of the remainder. The infiltrates of inbred animals with thyroiditis were demonstrated to contain cells capable of mediating antibody-dependent lymphoid cell-mediated cytotoxicity.
通过在完全弗氏佐剂中用甲状腺提取物进行同种免疫，在远交豚鼠中诱发实验性自身免疫性甲状腺炎。使用胶原酶和脱氧核糖核酸酶的消化程序，从患有重度甲状腺炎的动物组的汇集甲状腺中制备活的单细胞悬液。通过其与兔红细胞形成E花环的能力鉴定的胸腺来源或T淋巴细胞是主要的浸润淋巴细胞（75%）；骨髓来源或B细胞构成其余的大部分。已证明患有甲状腺炎的近交动物的浸润物中含有能够介导抗体依赖性淋巴细胞介导的细胞毒性的细胞。
