Aebischer P, Goddard M, Signore A P, Timpson R L
Section of Artifical Organs, Biomaterials and Cellular Technology, Brown University, Providence, Rhode Island 02912.
Exp Neurol. 1994 Apr;126(2):151-8. doi: 10.1006/exnr.1994.1053.
Cross-species neural grafting of cell lines immunoisolated by a permselective polymer membrane represents a promising source of neuroactive molecules for the treatment of neurodegenerative diseases. Utilization of a cell line of xenogeneic origin is advantageous since the transplanted cells will be rejected by the host immune system in case of breakage of the immunoisolating envelope. Polymer-encapsulated PC12 cells, a dopaminergic cell line derived from a rat pheochromocytoma, were transplanted in five Macaca fascicularis monkeys which had been previously rendered hemiparkinsonian by a unilateral carotid injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Well-preserved, tyrosine hydroxylase positive encapsulated PC12 cells were observed in the lesioned striatum for up to 5 months after implantation. Four out of five monkeys which received polymer-encapsulated PC12 cells showed significant behavioral improvement, whereas three monkeys implanted with either encapsulated bovine chromaffin cells or empty polymer envelopes showed no amelioration.
通过选择性通透聚合物膜免疫隔离的细胞系进行跨物种神经移植，是治疗神经退行性疾病的一种有前景的神经活性分子来源。使用异种来源的细胞系具有优势，因为如果免疫隔离包膜破裂，移植的细胞会被宿主免疫系统排斥。聚合物包裹的PC12细胞是一种源自大鼠嗜铬细胞瘤的多巴胺能细胞系，被移植到五只食蟹猴体内，这些猴子先前通过单侧颈动脉注射1-甲基-4-苯基-1,2,3,6-四氢吡啶而导致偏侧帕金森病。植入后长达5个月，在受损纹状体内观察到保存良好的、酪氨酸羟化酶阳性的包裹PC12细胞。接受聚合物包裹PC12细胞的五只猴子中有四只表现出明显的行为改善，而植入包裹牛嗜铬细胞或空聚合物包膜的三只猴子则没有改善。
