Campbell D G, Li P, Stephenson A J, Oakeshott R D
Orthopaedic Surgery Unit, Royal Adelaide Hospital North Terrace, Australia.
Int Orthop. 1994 Jun;18(3):172-6. doi: 10.1007/BF00192474.
A human immuno-deficiency virus (HIV) infected bone allograft model has been created using HTLV-IIIB virus in a concentration simulating a massively HIV infected bone allograft donor [HTLV-III is the denomination initially given to the human immuno-deficiency by the american team of Prof. Gallo. It represent the virus HIV 1 of the present international nomenclature]. 5 x 10(4) tissue culture infective doses per ml. of virus were placed within the medullary cavity of bovine femora and tibiae with a radiation dosimeter, and the ends sealed with lead. The bone/virus model was maintained at -70 degrees C while being irradiated with 1 to 4 megarads of gamma irradiation in increments of 0.5 megarads. The study showed that the HTLV-IIIB virus is a relatively radio-resistant organism, a property common to most viruses. The results suggest that HTLV-IIIB can be inactivated in bone infected with a clinically significant viral load, as may be found in donors who are initially negative when screened for HIV. It is recommended that bone allografts which are secondarily sterilized by gamma irradiation receive at least 2.5 megarads. The amount of radiation absorbed by the bone cortex was minimal.
已使用HTLV-IIIB病毒创建了一种人类免疫缺陷病毒（HIV）感染的骨移植模型，其浓度模拟大量HIV感染的骨移植供体[HTLV-III是加洛教授的美国团队最初赋予人类免疫缺陷病毒的名称。它代表当前国际命名法中的HIV-1病毒]。每毫升5×10⁴组织培养感染剂量的病毒用辐射剂量计置于牛股骨和胫骨的髓腔内，两端用铅密封。骨/病毒模型在-70℃下保存，同时以0.5兆拉德的增量接受1至4兆拉德的γ射线照射。研究表明，HTLV-IIIB病毒是一种相对抗辐射的生物体，这是大多数病毒共有的特性。结果表明，HTLV-IIIB可在临床显著病毒载量感染的骨中被灭活，如在HIV筛查最初为阴性的供体中可能发现的那样。建议通过γ射线照射进行二次灭菌的骨移植接受至少2.5兆拉德的照射。骨皮质吸收的辐射量最小。
