Fideler B M, Vangsness C T, Moore T, Li Z, Rasheed S
Department of Orthopaedic Surgery, University of Southern California School of Medicine, Los Angeles 90033.
J Bone Joint Surg Am. 1994 Jul;76(7):1032-5. doi: 10.2106/00004623-199407000-00011.
We studied the effects of several different doses of gamma radiation, ranging from 20,000 to 40,000 gray (2.0 to 4.0 megarad), with respect to the inactivation of the human immunodeficiency virus in fresh-frozen, whole bone-patellar ligament-bone grafts. Although the International Atomic Energy Agency has recommended the use of 25,000 gray of gamma radiation for the sterilization of medical products, the dose required for the inactivation of the human immunodeficiency virus in frozen allografts has not been established. Using one of the most sensitive and specific tests for the detection of the human immunodeficiency virus, the polymerase-chain-reaction test, we found that doses of 20,000 or 25,000 gray of gamma radiation did not destroy the genes of the human immunodeficiency virus effectively; DNA of the virus was detectable in the DNA of bone-marrow tissue obtained from grafts treated with these doses. However, DNA of the human immunodeficiency virus was not detectable in the grafts treated with 30,000 or 40,000 gray of gamma radiation. We conclude that a dose of 30,000 gray of gamma radiation or more is necessary for the sterilization of a fresh-frozen bone-patellar ligament-bone allograft, so that it can be used for reconstructive procedures without the risk of transmission of the virus to the recipient.
我们研究了几种不同剂量的γ射线辐射（范围从20,000至40,000戈瑞[2.0至4.0兆拉德]）对新鲜冷冻的全骨-髌韧带-骨移植物中人类免疫缺陷病毒灭活的影响。尽管国际原子能机构已推荐使用25,000戈瑞的γ射线辐射来对医疗产品进行灭菌，但冷冻同种异体移植物中人类免疫缺陷病毒灭活所需的剂量尚未确定。我们使用检测人类免疫缺陷病毒最灵敏且特异的检测方法之一——聚合酶链反应检测，发现20,000或25,000戈瑞的γ射线辐射剂量并不能有效破坏人类免疫缺陷病毒的基因；在用这些剂量处理过的移植物所获取的骨髓组织的DNA中可检测到病毒的DNA。然而，在用30,000或40,000戈瑞γ射线辐射处理过的移植物中未检测到人类免疫缺陷病毒的DNA。我们得出结论，对于新鲜冷冻的骨-髌韧带-骨同种异体移植物进行灭菌，需要30,000戈瑞或更高剂量的γ射线辐射，以便其可用于重建手术而无病毒传播给接受者的风险。
