Javaheri S, Corbett W, Adams J M, Davis P J, Gartside P S
Department of Veterans Affairs Medical Center, University of Cincinnati College of Medicine, Ohio 45220.
J Appl Physiol (1985). 1994 Jun;76(6):2651-5. doi: 10.1152/jappl.1994.76.6.2651.
NaCl cotransport carrier is known to be involved in transepithelial fluid absorption and secretion in various tissues. Recent studies indicate that Na-K-2Cl cotransport carrier also exists in the choroid plexus cells and that inhibition of the carrier decreases cerebrospinal fluid (CSF) production. In this study, we used large-dose intravenous furosemide, an inhibitor of Na-K-2Cl carrier, to determine the effects on cisternal CSF ionic composition in acute respiratory acidosis. In pentobarbital-anesthetized mechanically ventilated dogs, renal pedicles were ligated to prevent furosemide-induced diuresis. The experimental group (group II, n = 7) received 400 mg/kg of furosemide intravenously, and group I (control group, n = 7) received the vehicle. In group II, serial serum and CSF furosemide concentrations were approximately 10(-3) and 10(-5) mol/l, respectively. During 5 h of acute respiratory acidosis in both groups, the mean arterial PCO2 increased approximately 25 Torr, with comparable changes in CSF PCO2. In both groups, CSF [HCO3-] and [H+] rose approximately 3 meq/l and 20 neq/l, respectively. Changes in CSF [Na+], [K+], [Cl-], and [Na(+)-Cl-] were also similar and were not significantly different from each other when the two groups were compared. These data show that furosemide at the dose that inhibits NaCl cotransport carrier does not significantly alter ionic composition of cisternal CSF.
已知氯化钠共转运载体参与多种组织中的跨上皮液体吸收和分泌。最近的研究表明，钠 - 钾 - 2氯共转运载体也存在于脉络丛细胞中，并且抑制该载体可减少脑脊液（CSF）的产生。在本研究中，我们使用大剂量静脉注射速尿（一种钠 - 钾 - 2氯载体抑制剂）来确定其对急性呼吸性酸中毒时脑池脑脊液离子成分的影响。在戊巴比妥麻醉、机械通气的犬中，结扎肾蒂以防止速尿诱导的利尿。实验组（II组，n = 7）静脉注射400mg / kg速尿，I组（对照组，n = 7）注射溶媒。在II组中，血清和脑脊液速尿的系列浓度分别约为10^(-3)和10^(-5)mol / l。在两组急性呼吸性酸中毒的5小时期间，平均动脉血PCO2增加约25 Torr，脑脊液PCO2有类似变化。两组中，脑脊液[HCO3-]和[H+]分别升高约3 meq / l和20 neq / l。脑脊液[Na+]、[K+]、[Cl-]和[Na(+)-Cl-]的变化也相似，两组比较时彼此无显著差异。这些数据表明，抑制氯化钠共转运载体的剂量的速尿不会显著改变脑池脑脊液的离子成分。
