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J Bacteriol. 1994 Oct;176(19):5958-61. doi: 10.1128/jb.176.19.5958-5961.1994.
2
Role of the origin of transfer in termination of strand transfer during bacterial conjugation.转移起始点在细菌接合过程中链转移终止中的作用。
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Nucleic Acids Res. 1993 Sep 25;21(19):4563-8. doi: 10.1093/nar/21.19.4563.
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Genetic organization of plasmid R1162 DNA involved in conjugative mobilization.参与接合转移的质粒R1162 DNA的遗传组织。
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The origin of greater-than-unit-length plasmids generated during bacterial conjugation.细菌接合过程中产生的大于单位长度质粒的起源。
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Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the staphylococcal plasmid pGO1.葡萄球菌质粒pGO1上接合转移起始点（oriT）及编码单链核酸内切酶的基因（nes）的起源鉴定与特性分析
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Origin and fate of the 3' ends of single-stranded DNA generated by conjugal transfer of plasmid R1162.由质粒 R1162 接合转移产生的单链 DNA 3'末端的起源和命运。
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2
TraA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution.革兰氏阳性质粒pIP501的TraA及其N端松弛酶结构域表现出特异性oriT结合，并在溶液中以二聚体形式存在。
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Molecular handcuffing of the relaxosome at the origin of conjugative transfer of the plasmid R1162.质粒R1162接合转移起始点处松弛体的分子束缚作用
Nucleic Acids Res. 2003 Aug 15;31(16):4762-8. doi: 10.1093/nar/gkg687.
4
The primase of broad-host-range plasmid R1162 is active in conjugal transfer.广宿主范围质粒R1162的引发酶在接合转移中具有活性。
J Bacteriol. 1996 Dec;178(23):6888-94. doi: 10.1128/jb.178.23.6888-6894.1996.
5
MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA.MobB蛋白通过增加复合质粒DNA的比例来刺激在转移起点R1162处的切口形成。
J Bacteriol. 1996 Oct;178(19):5762-7. doi: 10.1128/jb.178.19.5762-5767.1996.

本文引用的文献

1
Initiation and termination of DNA transfer at F plasmid oriT.F质粒oriT处DNA转移的起始与终止
Mol Microbiol. 1994 Feb;11(3):449-58. doi: 10.1111/j.1365-2958.1994.tb00326.x.
2
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
3
Temperature-sensitive copy number mutants of CoIE1 are located in an untranslated region of the plasmid genome.ColE1的温度敏感型拷贝数突变体位于质粒基因组的一个非翻译区域。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3570-4. doi: 10.1073/pnas.79.11.3570.
4
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.质粒ColE1作为用于DNA克隆和扩增的分子载体。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9. doi: 10.1073/pnas.71.9.3455.
5
A 38 base-pair segment of DNA is required in cis for conjugative mobilization of broad host-range plasmid R1162.广泛宿主范围质粒R1162的接合转移需要一段38个碱基对的DNA顺式作用片段。
J Mol Biol. 1987 Dec 5;198(3):361-9. doi: 10.1016/0022-2836(87)90286-5.
6
Unidirectional transfer of broad host-range plasmid R1162 during conjugative mobilization. Evidence for genetically distinct events at oriT.接合转移过程中广宿主范围质粒R1162的单向转移。oriT处基因不同事件的证据。
J Mol Biol. 1989 Aug 5;208(3):501-5. doi: 10.1016/0022-2836(89)90513-5.
7
Site-specific recombination at oriT of plasmid R1162 in the absence of conjugative transfer.在不存在接合转移的情况下，质粒R1162 oriT处的位点特异性重组。
J Bacteriol. 1989 Feb;171(2):799-806. doi: 10.1128/jb.171.2.799-806.1989.
8
Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand.
Nucleic Acids Res. 1990 Jun 25;18(12):3579-86. doi: 10.1093/nar/18.12.3579.
9
A segment of a plasmid gene required for conjugal transfer encodes a site-specific, single-strand DNA endonuclease and ligase.
Nucleic Acids Res. 1991 Mar 11;19(5):1129-37. doi: 10.1093/nar/19.5.1129.
10
Role of the origin of transfer in termination of strand transfer during bacterial conjugation.转移起始点在细菌接合过程中链转移终止中的作用。
J Bacteriol. 1992 Oct;174(20):6659-65. doi: 10.1128/jb.174.20.6659-6665.1992.

质粒DNA的接合转移：质粒R1162转移起点处的终止频率

Conjugal mobilization of plasmid DNA: termination frequency at the origin of transfer of plasmid R1162.

作者信息

Rao X M, Meyer R J

机构信息

Department of Microbiology, University of Texas, Austin 78712.

出版信息

J Bacteriol. 1994 Oct;176(19):5958-61. doi: 10.1128/jb.176.19.5958-5961.1994.

DOI:10.1128/jb.176.19.5958-5961.1994
PMID:7928956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196812/
Abstract

Conjugal transfer of plasmid R1162 is initiated and terminated at a 38-bp origin of transfer (oriT). Plasmids containing two directly repeated copies of oriT were used to determine the actual frequency of termination at this site. This frequency was calculated both for oriTnic, a mutated origin that cannot act as the initiation site of transfer, and for an unmutated oriT where transfer had been initiated. In both cases, the termination frequency decreased as the distance between the initiation and termination sites became greater and was significantly less than one for plasmids the size of R1162. A substantial proportion of recipient cells received more than one plasmid copy during transfer. Our results indicate that termination is inefficient but that this is partly compensated for by the transmission of multiple plasmid copies.

摘要

质粒R1162的接合转移在一个38碱基对的转移起始点（oriT）起始和终止。含有两个直接重复oriT拷贝的质粒被用于确定该位点实际的终止频率。针对oriTnic（一个不能作为转移起始位点的突变起始点）和一个已起始转移的未突变oriT，都计算了终止频率。在这两种情况下，终止频率都随着起始位点和终止位点之间的距离增大而降低，并且对于R1162大小的质粒而言，终止频率显著小于1。相当一部分受体细胞在转移过程中接收了不止一个质粒拷贝。我们的结果表明，终止效率低下，但这在一定程度上通过多个质粒拷贝的传递得到了补偿。