• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Transmembrane pH of Clostridium acetobutylicum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased.当氢化酶的体内活性降低时，丙酮丁醇梭菌的跨膜pH值会反转（内部酸性更强）。
J Bacteriol. 1994 Oct;176(19):6146-7. doi: 10.1128/jb.176.19.6146-6147.1994.
2
Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures.丁酸梭菌VPI 3266在葡萄糖-甘油混合物上生长时碳流和电子流的调控
J Bacteriol. 2001 Mar;183(5):1748-54. doi: 10.1128/JB.183.5.1748-1754.2001.
3
Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum during growth under acetogenic and solventogenic conditions.产乙酸和产溶剂条件下丙酮丁醇梭菌生长过程中的跨膜pH梯度和膜电位
Appl Environ Microbiol. 1985 Oct;50(4):1043-7. doi: 10.1128/aem.50.4.1043-1047.1985.
4
Unleashing hydrogenase activity in carbon monoxide dehydrogenase/acetyl-CoA synthase and pyruvate:ferredoxin oxidoreductase.释放一氧化碳脱氢酶/乙酰辅酶A合成酶和丙酮酸：铁氧化还原蛋白氧化还原酶中的氢化酶活性。
Biochemistry. 1996 Dec 10;35(49):15814-21. doi: 10.1021/bi9615598.
5
Effect of acidic pH on the ability of Clostridium sporogenes MD1 to take up and retain intracellular potassium.酸性pH对生孢梭菌MD1摄取和保留细胞内钾能力的影响。
FEMS Microbiol Lett. 2007 Feb;267(1):46-50. doi: 10.1111/j.1574-6968.2006.00535.x.
6
Molecular dynamics study of the proposed proton transport pathways in [FeFe]-hydrogenase.对[铁铁]氢化酶中质子传输途径的分子动力学研究。
Biochim Biophys Acta. 2014 Jan;1837(1):131-8. doi: 10.1016/j.bbabio.2013.08.004. Epub 2013 Aug 24.
7
The ability of acidic pH, growth inhibitors, and glucose to increase the proton motive force and energy spilling of amino acid-fermenting Clostridium sporogenes MD1 cultures.酸性pH值、生长抑制剂和葡萄糖增加产芽孢氨基酸发酵型梭状芽孢杆菌MD1培养物的质子动力和能量溢流的能力。
Arch Microbiol. 2005 May;183(4):236-42. doi: 10.1007/s00203-005-0765-x. Epub 2005 Mar 15.
8
Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners.丙酮丁醇梭菌[FeFe]-氢化酶的完整活性概况及内源性氧化还原伙伴的动力学参数。
FEMS Microbiol Lett. 2007 Oct;275(1):113-21. doi: 10.1111/j.1574-6968.2007.00868.x. Epub 2007 Aug 6.
9
Electron transfer activation of a second water channel for proton transport in [FeFe]-hydrogenase.[铁铁]氢化酶中用于质子传输的第二个水通道的电子转移激活。
J Chem Phys. 2014 Dec 14;141(22):22D527. doi: 10.1063/1.4902236.
10
Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers.在与含有细菌反应中心的脂质体融合的嗜热链球菌或丙酮丁醇梭菌膜囊泡中，光驱动的氨基酸摄取。
J Bacteriol. 1988 Apr;170(4):1820-4. doi: 10.1128/jb.170.4.1820-1824.1988.

引用本文的文献

1
Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia.通过人工微生物群落的精确设计，超越 Thauer 极限生产生物氢。
Commun Biol. 2020 Aug 14;3(1):443. doi: 10.1038/s42003-020-01159-x.
2
Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824.木质素对丙酮丁醇梭菌ATCC 824生物燃料生产影响的定量蛋白质组学分析
Biotechnol Biofuels. 2016 May 31;9:113. doi: 10.1186/s13068-016-0523-0. eCollection 2016.
3
Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum.丙酮丁醇梭菌对氧化己糖衍生物的发酵作用
Microb Cell Fact. 2014 Sep 18;13:139. doi: 10.1186/s12934-014-0139-7.
4
Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum.用于丙酮丁醇梭菌的灵敏基因表达报告系统和诱导型启动子-阻遏系统的开发。
Appl Environ Microbiol. 2003 Aug;69(8):4985-8. doi: 10.1128/AEM.69.8.4985-4988.2003.
5
Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures.丁酸梭菌VPI 3266在葡萄糖-甘油混合物上生长时碳流和电子流的调控
J Bacteriol. 2001 Mar;183(5):1748-54. doi: 10.1128/JB.183.5.1748-1754.2001.
6
Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824.丙酮丁醇梭菌ATCC 824推定氢化酶基因的分子特征及转录分析
J Bacteriol. 1996 May;178(9):2668-75. doi: 10.1128/jb.178.9.2668-2675.1996.

本文引用的文献

1
Reduced coenzyme F420: heterodisulfide oxidoreductase, a proton- translocating redox system in methanogenic bacteria.还原型辅酶F420：异二硫键氧化还原酶，一种产甲烷菌中的质子转运氧化还原系统。
Proc Natl Acad Sci U S A. 1990 Dec 1;87(23):9449-53. doi: 10.1073/pnas.87.23.9449.
2
Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol.在中性pH值下，以葡萄糖和甘油混合物为底物，在恒化器培养中生长的丙酮丁醇梭菌中碳流和电子流的调控
J Bacteriol. 1994 Mar;176(5):1443-50. doi: 10.1128/jb.176.5.1443-1450.1994.
3
Hydrogenase.氢化酶
Biochim Biophys Acta. 1980 Dec;594(2-3):105-76. doi: 10.1016/0304-4173(80)90007-5.
4
Relationship between phosphorylation potential and electrochemical H+ gradient during glycolysis in Streptococcus lactis.乳酸链球菌糖酵解过程中磷酸化电位与电化学H⁺梯度之间的关系。
J Bacteriol. 1983 Mar;153(3):1461-70. doi: 10.1128/jb.153.3.1461-1470.1983.
5
Interconversion of components of the bacterial proton motive force by electrogenic potassium transport.通过生电钾转运实现细菌质子动力势各组分的相互转化。
J Bacteriol. 1981 Sep;147(3):820-6. doi: 10.1128/jb.147.3.820-826.1981.
6
Stoichiometry of the H+-ATPase of Escherichia coli cells during anaerobic growth.
FEBS Lett. 1983 Apr 18;154(2):343-6. doi: 10.1016/0014-5793(83)80179-3.
7
Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate-reducing bacteria Desulfovibrio.脱硫弧菌中氢化酶、电子传递蛋白与能量偶联
Annu Rev Microbiol. 1984;38:551-92. doi: 10.1146/annurev.mi.38.100184.003003.
8
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation.氧化磷酸化和光合磷酸化中的化学渗透偶联
Biol Rev Camb Philos Soc. 1966 Aug;41(3):445-502. doi: 10.1111/j.1469-185x.1966.tb01501.x.
9
Low-affinity potassium uptake system in Bacillus acidocaldarius.嗜酸热硫化叶菌中的低亲和力钾离子吸收系统。
J Bacteriol. 1987 Sep;169(9):4335-41. doi: 10.1128/jb.169.9.4335-4341.1987.
10
The proton motive force in bacteria: a critical assessment of methods.细菌中的质子动力势：方法的批判性评估
Annu Rev Microbiol. 1985;39:219-42. doi: 10.1146/annurev.mi.39.100185.001251.

当氢化酶的体内活性降低时，丙酮丁醇梭菌的跨膜pH值会反转（内部酸性更强）。

Transmembrane pH of Clostridium acetobutylicum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased.

作者信息

Girbal L, Vasconcelos I, Soucaille P

机构信息

Centre de Ingénierie Gilbert Durand, Institut National des Sciences Appliquées, Toulouse, France.

出版信息

J Bacteriol. 1994 Oct;176(19):6146-7. doi: 10.1128/jb.176.19.6146-6147.1994.

DOI:10.1128/jb.176.19.6146-6147.1994
PMID:7928980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196838/
Abstract

Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a constant proton motive force by interconverting chemical and electrical potentials.

摘要

本文报道的证据表明，丙酮丁醇梭菌细胞质的碱化涉及氢化酶活性。体内氢化酶活性的降低伴随着质子在细胞内的积累，导致负的（内部酸性）pH梯度。然而，该生物体能够通过化学势和电势的相互转换来维持恒定的质子动力。