当氢化酶的体内活性降低时，丙酮丁醇梭菌的跨膜pH值会反转（内部酸性更强）。
Transmembrane pH of Clostridium acetobutylicum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased.
作者信息
Girbal L, Vasconcelos I, Soucaille P
机构信息
Centre de Ingénierie Gilbert Durand, Institut National des Sciences Appliquées, Toulouse, France.
出版信息
J Bacteriol. 1994 Oct;176(19):6146-7. doi: 10.1128/jb.176.19.6146-6147.1994.
Abstract
Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a constant proton motive force by interconverting chemical and electrical potentials.
摘要
本文报道的证据表明，丙酮丁醇梭菌细胞质的碱化涉及氢化酶活性。体内氢化酶活性的降低伴随着质子在细胞内的积累，导致负的（内部酸性）pH梯度。然而，该生物体能够通过化学势和电势的相互转换来维持恒定的质子动力。
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本文引用的文献
1
Reduced coenzyme F420: heterodisulfide oxidoreductase, a proton- translocating redox system in methanogenic bacteria.还原型辅酶F420：异二硫键氧化还原酶，一种产甲烷菌中的质子转运氧化还原系统。
Proc Natl Acad Sci U S A. 1990 Dec 1;87(23):9449-53. doi: 10.1073/pnas.87.23.9449.
2
Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol.在中性pH值下，以葡萄糖和甘油混合物为底物，在恒化器培养中生长的丙酮丁醇梭菌中碳流和电子流的调控
J Bacteriol. 1994 Mar;176(5):1443-50. doi: 10.1128/jb.176.5.1443-1450.1994.
3
Hydrogenase.氢化酶
Biochim Biophys Acta. 1980 Dec;594(2-3):105-76. doi: 10.1016/0304-4173(80)90007-5.
4
Relationship between phosphorylation potential and electrochemical H+ gradient during glycolysis in Streptococcus lactis.乳酸链球菌糖酵解过程中磷酸化电位与电化学H⁺梯度之间的关系。
J Bacteriol. 1983 Mar;153(3):1461-70. doi: 10.1128/jb.153.3.1461-1470.1983.
5
Interconversion of components of the bacterial proton motive force by electrogenic potassium transport.通过生电钾转运实现细菌质子动力势各组分的相互转化。
J Bacteriol. 1981 Sep;147(3):820-6. doi: 10.1128/jb.147.3.820-826.1981.
6
Stoichiometry of the H+-ATPase of Escherichia coli cells during anaerobic growth.
FEBS Lett. 1983 Apr 18;154(2):343-6. doi: 10.1016/0014-5793(83)80179-3.
7
Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate-reducing bacteria Desulfovibrio.脱硫弧菌中氢化酶、电子传递蛋白与能量偶联
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8
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Biol Rev Camb Philos Soc. 1966 Aug;41(3):445-502. doi: 10.1111/j.1469-185x.1966.tb01501.x.
9
Low-affinity potassium uptake system in Bacillus acidocaldarius.嗜酸热硫化叶菌中的低亲和力钾离子吸收系统。
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10
The proton motive force in bacteria: a critical assessment of methods.细菌中的质子动力势：方法的批判性评估
Annu Rev Microbiol. 1985;39:219-42. doi: 10.1146/annurev.mi.39.100185.001251.
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