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吐温20能使心磷脂从酶联免疫吸附测定（ELISA）板上分离，无论是否存在β2-糖蛋白I，均可使抗心磷脂抗体无法检测到。

Tween 20 detaches cardiolipin from ELISA plates and makes anticardiolipin antibodies undetectable regardless of the presence of beta 2-glycoprotein-I.

作者信息

Cabral A R, Cabiedes J, Alarcón-Segovia D

机构信息

Department of Immunology and Rheumatology, Instituto Nacional de la Nutrición Salvador Zubirán, Tlalpan, Mexico.

出版信息

J Immunol Methods. 1994 Sep 30;175(1):107-14. doi: 10.1016/0022-1759(94)90336-0.

DOI:10.1016/0022-1759(94)90336-0
PMID:7930632
Abstract

We investigated the effects of polyoxyethylene sorbitan monolaurate (Tween 20) in the detection of IgG anticardiolipin antibodies (aCL) by the CL-beta 2-glycoprotein-I and the standard aCL solid-phase immunoassays. We found that Tween 20 disengages cardiolipin from a variety of microtiter wells rendering aCL undetectable by both methods. Our results agree with a previous report but are discordant with others. We offer rationale that may explain some of the discrepancies. Based in our findings, we do not recommend the use of Tween 20 for the detection of aCL.

摘要

我们通过CL-β2-糖蛋白-I和标准抗心磷脂（aCL）固相免疫测定法，研究了聚氧乙烯山梨醇酐单月桂酸酯（吐温20）在检测IgG抗心磷脂抗体（aCL）中的作用。我们发现吐温20会使心磷脂从各种微量滴定孔中脱离，导致两种方法均无法检测到aCL。我们的结果与之前的一份报告一致，但与其他报告不一致。我们提供了可能解释部分差异的理由。基于我们的研究结果，我们不建议使用吐温20来检测aCL。

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引用本文的文献

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Testing for and clinical significance of anticardiolipin antibodies.抗心磷脂抗体的检测及其临床意义
Clin Diagn Lab Immunol. 1999 Nov;6(6):775-82. doi: 10.1128/CDLI.6.6.775-782.1999.
3
Antiphospholipid antibodies are directed against epitopes of oxidized phospholipids. Recognition of cardiolipin by monoclonal antibodies to epitopes of oxidized low density lipoprotein.
抗磷脂抗体针对氧化磷脂的表位。单克隆抗体对氧化低密度脂蛋白表位的心磷脂识别。
J Clin Invest. 1996 Aug 1;98(3):815-25. doi: 10.1172/JCI118854.