用巨噬细胞粘附抑制试验检测针对蛋白质抗原的细胞介导免疫（作者译）
[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)].
作者信息
Schimke R, Eichler C, Ambrosius H
出版信息
Allerg Immunol (Leipz). 1978;24(1):61-9.
The macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (BGG) in guinea pigs. The test was performed with peritoneal cells (PC) in hemocytometer chambers. 10 mg/ml BGG were added as antigen. The washing step was performed with a burette. An average standard deviation of 5% was obtained with this washing method. Day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated immunity against BGG. Supernatants of sensitized spleen cells or lymph node cells produce adherence inhibition in PC of nonimmunized guinea pigs too.
巨噬细胞黏附抑制试验是检测豚鼠针对牛γ球蛋白（BGG）的细胞介导免疫的合适检测方法。该试验在血细胞计数板中用腹腔细胞（PC）进行。加入10mg/ml BGG作为抗原。洗涤步骤用滴定管进行。用这种洗涤方法获得的平均标准差为5%。对个体动物的逐日研究表明，针对BGG的细胞介导免疫程度存在差异。致敏脾细胞或淋巴结细胞的上清液也会在未免疫豚鼠的PC中产生黏附抑制。
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