Hill F S, Cox A B, Salmon Y L, Cantu A O, Lucas J N
Lawrence Livermore National Laboratory, University of California, Livermore 94551.
Int J Radiat Biol. 1994 Oct;66(4):381-4. doi: 10.1080/09553009414551321.
The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological dosimetry studies. We show that staphylococcal enterotoxin A (SEA), one of the most potent mitogens known, at a concentration of 0.5 microgram/ml stimulated peripheral lymphocytes to grow with a mitotic index (MI) averaging 0.13 metaphases/cell in old, irradiated rhesus macaques. This was significantly greater (p < 0.001) than that produced by PHA (MI < 0.01) in lymphocytes from the same animals. Whole blood was cultured for 96, 120 and 144 h for five irradiated individuals and for two controls. All cells cultured with SEA produced a high MI with a peak response at 120 h whereas the same cultures showed low MI for each PHA stimulated culture.
促有丝分裂原植物血凝素（PHA）在人类和食蟹猴（猕猴）淋巴细胞培养中均能有效刺激T细胞增殖。恒河猴（恒河猴）的T细胞反应性低于人类细胞，例如在生物剂量学研究中，当需要数千个中期相时，产生的中期相很少。我们发现，已知最强效的促有丝分裂原之一葡萄球菌肠毒素A（SEA），在浓度为0.5微克/毫升时，能刺激老年、受过辐照的恒河猴外周淋巴细胞生长，有丝分裂指数（MI）平均为0.13个中期相/细胞。这显著高于（p < 0.001）同一动物淋巴细胞中PHA产生的有丝分裂指数（MI < 0.01）。对5名受过辐照的个体和2名对照个体的全血进行了96、120和144小时的培养。所有用SEA培养的细胞产生了高有丝分裂指数，在120小时达到峰值反应，而相同培养物中PHA刺激的培养物有丝分裂指数较低。
