一种用于评估针对主要组织相容性复合体抗原的同种抗体的溶血斑试验。
A haemolytic spot test for the estimation of alloantibody against antigens of the major histocompatibility complex.
作者信息
Jensenius J C
出版信息
Transplantation. 1976 Nov;22(5):415-9. doi: 10.1097/00007890-197611000-00001.
Abstract
A haemolytic technique for the assay of alloantibody has been developed. Titration of alloantiserum was accomplished by placing microdroplets of serum dilutions on agarose layers containing the appropriate erythrocytes. Complement-mediated lysis was procured after the addition of rabbit serum. The assay appeared specific for products of the major histocompatibility gene complex in the rat and in the mouse.
摘要
已开发出一种用于检测同种抗体的溶血技术。通过将血清稀释液的微滴置于含有适当红细胞的琼脂糖层上来完成同种抗血清的滴定。加入兔血清后可实现补体介导的溶血。该检测方法似乎对大鼠和小鼠主要组织相容性基因复合体的产物具有特异性。
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