A haemolytic spot test for the estimation of alloantibody against antigens of the major histocompatibility complex.

A haemolytic technique for the assay of alloantibody has been developed. Titration of alloantiserum was accomplished by placing microdroplets of serum dilutions on agarose layers containing the appropriate erythrocytes. Complement-mediated lysis was procured after the addition of rabbit serum. The assay appeared specific for products of the major histocompatibility gene complex in the rat and in the mouse.