Huang M E, Chuat J C, Galibert F
Laboratoire de Biochemie et Biologie Moleculaire, Faculté de Médecine, Cedex, France.
J Mol Biol. 1994 Sep 30;242(4):595-8. doi: 10.1006/jmbi.1994.1607.
We report the sequencing and identification on chromosome X of Saccharomyces cerevisiae of an open reading frame whose product, designated yClC-1, displays significant structural similarity to a voltage-gated Cl- channel family. This putative protein contains 13 hydrophobic domains very similar to transmembrane domains exhibited by known members of this family. Some amino acids in the domains and at the loops between them are well conserved among all members. This is the first voltage-gated Cl- channel described in the yeast S. cerevisiae. The identification of yClC-1 will facilitate the functional analysis of Cl- channels in general, and should also assist in the identification of other ClC genes in higher eukaryotes.
我们报告了对酿酒酵母X染色体上一个开放阅读框的测序和鉴定，其产物命名为yClC-1，与电压门控氯离子通道家族具有显著的结构相似性。这个推定的蛋白质包含13个疏水结构域，与该家族已知成员展示的跨膜结构域非常相似。这些结构域及其之间环上的一些氨基酸在所有成员中都高度保守。这是在酿酒酵母中描述的第一个电压门控氯离子通道。yClC-1的鉴定将有助于一般氯离子通道的功能分析，也应有助于在高等真核生物中鉴定其他ClC基因。
