Smits P H, De Haan M, Maat C, Grivell L A
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Yeast. 1994 Apr;10 Suppl A:S75-80. doi: 10.1002/yea.320100010.
We report here the sequence of a 33,117 bp DNA fragment located approximately 30 kb from the centromere on the right arm of Saccharomyces cerevisiae chromosome II. We have detected 16 open reading frames (ORFs) longer than 450 bp, provisionally called YBR0301 to YBR0322, covering 70.4% of the entire sequence. The ORFs YBR0301, YBR0302, YBR0303, YBR0305 and YBR0315 correspond to previously sequenced S. cerevisiae genes GAL10, GAL1, FUR4, CAL1 and L2B, respectively. Translation products of two other ORFs, YBR0308 and YBR0312 exhibit similarity to previously known S. cerevisiae proteins: the mitochondrially associated protein SCO1 and the protein kinase YKR2. The predicted protein product of the ORF YBR0321 shows a 41.6% identity score with the Escherichia coli pyroxamine 5'-phosphate oxidase. The nine other ORFs show no significant homology to known proteins.
我们在此报告位于酿酒酵母二号染色体右臂着丝粒约30 kb处的一段33,117 bp DNA片段的序列。我们检测到16个长度超过450 bp的开放阅读框（ORF），暂命名为YBR0301至YBR0322，覆盖了整个序列的70.4%。ORF YBR0301、YBR0302、YBR0303、YBR0305和YBR0315分别对应于先前测序的酿酒酵母基因GAL10、GAL1、FUR4、CAL1和L2B。另外两个ORF，YBR0308和YBR0312的翻译产物与先前已知的酿酒酵母蛋白具有相似性：线粒体相关蛋白SCO1和蛋白激酶YKR2。ORF YBR0321的预测蛋白产物与大肠杆菌吡哆胺5'-磷酸氧化酶的同一性得分是41.6%。其他九个ORF与已知蛋白没有明显的同源性。
