Pineda M H, Dooley M P
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames 50011-1250.
J Vet Pharmacol Ther. 1994 Jun;17(3):169-74. doi: 10.1111/j.1365-2885.1994.tb00230.x.
This study was carried out to determine whether yohimbine antagonizes the retrograde flow of spermatozoa into the urinary bladder of dogs caused by xylazine. Adult dogs were assigned to one of four groups of six dogs each and treated as follows: saline control, xylazine (2.2 mg/kg, i.m.), yohimbine (0.2 mg/kg, i.m.), yohimbine/xylazine (yohimbine, 0.2 mg/kg, i.m., followed 10 min later by xylazine, 2.2 mg/kg, i.m.). Pre- and post-treatment urine were collected by cystocentesis from all dogs. The mean (+/- SD) adjusted total number of spermatozoa in the post-treatment urine of xylazine-treated dogs (141.02 +/- 136.75 x 10(6)) was 15 times higher (P < 0.05) than the number in the post-treatment urine of control dogs (9.16 +/- 20.26 x 10(6), 1763 times higher (P < 0.05) than the number in the urine of yohimbine-treated dogs (0.08 +/- 0.20 x 10(6), and 56 times higher (P < 0.05) than the total number in the post-treatment urine of yohimbine/xylazine-treated dogs (2.54 +/- 4.54 x 10(6)). These results confirm that xylazine induces a significant (P = 0.007) displacement of spermatozoa into the urinary bladder of dogs and demonstrate that pre-treatment with yohimbine prevents this effect.
本研究旨在确定育亨宾是否能拮抗由赛拉嗪引起的犬精子逆行流入膀胱的现象。成年犬被分为四组，每组六只，并进行如下处理：生理盐水对照、赛拉嗪（2.2毫克/千克，肌肉注射）、育亨宾（0.2毫克/千克，肌肉注射）、育亨宾/赛拉嗪（育亨宾，0.2毫克/千克，肌肉注射，10分钟后再注射赛拉嗪，2.2毫克/千克，肌肉注射）。通过膀胱穿刺术收集所有犬治疗前和治疗后的尿液。赛拉嗪治疗组犬治疗后尿液中精子平均（±标准差）调整总数（141.02±136.75×10⁶）比对照组犬治疗后尿液中的精子数（9.16±20.26×10⁶）高15倍（P<0.05），比育亨宾治疗组犬尿液中的精子数（0.08±0.20×10⁶）高1763倍（P<0.05），比育亨宾/赛拉嗪治疗组犬治疗后尿液中的总数（2.54±4.54×10⁶）高56倍（P<0.05）。这些结果证实赛拉嗪可导致犬精子显著（P = 0.007）移位至膀胱，并表明育亨宾预处理可防止这种效应。
