Dooley M P, Pineda M H, Hopper J G, Hsu W H
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames 50011.
Am J Vet Res. 1990 Oct;51(10):1574-9.
Retrograde flow of spermatozoa into the urinary bladder of dogs during ejaculation or after administration of xylazine was examined. In experiment 1, the mean (+/- SD) spermatozoal concentration in urine collected by cystocentesis before ejaculation was 0.322 +/- 0.645 X 10(6)/ml. After ejaculation, motile spermatozoa were present in the urine collected by cystocentesis from 12 of 15 dogs, and the concentration of spermatozoa in the urine (5.139 +/- 7.014 X 10(6)/ml) was higher (P less than 0.025) than the concentration in the urine collected before ejaculation. The percentage of the total number of spermatozoa that were displaced during ejaculation and flowed into the urinary bladder (retrograde flow) ranged from 0 to 99.75% (24.67 +/- 33.98%). In experiments 2 and 3, administration of xylazine to sexually rested dogs induced retrograde flow of spermatozoa into the urinary bladder. In experiment 2, all dogs had spermatozoa in urine collected after xylazine administration, with motile spermatozoa present in the urine from 9 of 10 dogs. In experiment 3, urine collected from dogs before administration of xylazine was azoospermic or contained few, nonmotile spermatozoa (0.063 +/- 0.135 X 10(6)/ml), whereas urine collected after administration of xylazine had more (P less than 0.025) and motile spermatozoa (3.717 +/- 4.273 X 10(6)/ml). In experiment 4, administration of xylazine to dogs after ejaculation did not increase the concentration of spermatozoa in the urine. Results indicate that spermatozoa flow into the urinary bladder of dogs during ejaculation or after administration of xylazine to sexually rested dogs.
对射精期间或给予赛拉嗪后狗的精子逆流至膀胱的情况进行了检查。在实验1中，射精前通过膀胱穿刺采集的尿液中精子平均浓度（±标准差）为0.322±0.645×10⁶/ml。射精后，15只狗中有12只通过膀胱穿刺采集的尿液中存在活动精子，且尿液中精子浓度（5.139±7.014×10⁶/ml）高于射精前采集尿液中的浓度（P＜0.025）。射精期间移位并流入膀胱（逆流）的精子总数百分比在0至99.75%（24.67±33.98%）之间。在实验2和3中，对性静止的狗给予赛拉嗪会导致精子逆流至膀胱。在实验2中，所有狗在给予赛拉嗪后采集的尿液中都有精子，10只狗中有9只尿液中存在活动精子。在实验3中，给予赛拉嗪前采集的狗尿液无精子或含有少量不活动精子（0.063±0.135×10⁶/ml），而给予赛拉嗪后采集的尿液中有更多（P＜0.025）且为活动精子（3.717±4.273×10⁶/ml）。在实验4中，射精后对狗给予赛拉嗪并未增加尿液中精子浓度。结果表明，射精期间或对性静止的狗给予赛拉嗪后精子会流入狗的膀胱。
