Dooley M P, Pineda M H, Hopper J G, Hsu W H
Department of Veterinary Physiology, College of Veterinary Medicine, Iowa State University, Ames 50011-1250.
Am J Vet Res. 1991 May;52(5):687-91.
The effect of methoxamine on retrograde flow of spermatozoa into the urinary bladder of domestic cats during electroejaculation and the incidence of retrograde flow during the collection of semen with an artificial vagina, or during mating was examined. In experiment 1, urine collected by cystocentesis prior to electroejaculation was azoospermic or contained few, nonmotile spermatozoa, whereas urine collected after electroejaculation contained more (P = 0.002) spermatozoa, and motile spermatozoa were evident in urine obtained from 6 of 8 cats. Administration of methoxamine hydrochloride (200 micrograms/kg of body weight, IV) did not affect spermatozoal output or percentage of retrograde flow. Percentage of retrograde flow for control cats ranged from 61.18 to 92.95% (mean +/- SD, 80.00 +/- 14.28%) and for methoxamine-treated cats, ranged from 15.25 to 92.49% (mean +/- SD, 58.10 +/- 32.28%), but the difference was not significant. In experiment 2, an artificial vagina was used to collect semen from 5 of the 8 cats used in experiment 1. Urine collected by cystocentesis after ejaculation contained spermatozoa, and motile spermatozoa were evident in the urine from 4 of 5 cats. The mean (+/- SD) percentage of retrograde flow for these 5 cats was 46.82 +/- 31.67% (range, 14.56 to 90.32%). In experiment 3, each of the 5 cats that were used in experiments 1 and 2 were mated. Spermatozoa were recovered from the vagina of each mated female, and motile spermatozoa were also present in postejaculation urine obtained by cystocentesis from each of the 5 male cats. Mean total number of spermatozoa in the postmating urine was 29.42 +/- 33.58 x 10(6) (range, 0.22 x 10(6) to 76.05 x 10(6) spermatozoa).(ABSTRACT TRUNCATED AT 250 WORDS)
研究了甲氧明对家猫电刺激采精时精子逆行流入膀胱的影响，以及在使用人工阴道采精或交配过程中逆行流的发生率。在实验1中，电刺激采精前通过膀胱穿刺采集的尿液无精子或仅含少量无活力精子，而电刺激采精后采集的尿液含有更多（P = 0.002）精子，并且在8只猫中的6只猫的尿液中可见活动精子。静脉注射盐酸甲氧明（200微克/千克体重）不影响精子输出或逆行流百分比。对照猫的逆行流百分比范围为61.18%至92.95%（平均值±标准差，80.00±14.28%），甲氧明处理的猫的逆行流百分比范围为15.25%至92.49%（平均值±标准差，58.10±32.28%），但差异不显著。在实验2中，使用人工阴道从实验1中的8只猫中的5只采集精液。射精后通过膀胱穿刺采集的尿液含有精子，并且在5只猫中的4只猫的尿液中可见活动精子。这5只猫的逆行流平均（±标准差）百分比为46.82±31.67%（范围为14.56%至90.32%）。在实验3中，将实验1和2中使用的5只猫分别进行交配。从每只交配母猫的阴道中回收了精子，并且通过膀胱穿刺从每只5只雄猫采集的射精后尿液中也存在活动精子。交配后尿液中精子的平均总数为29.42±33.58×10⁶（范围为0.22×10⁶至76.05×10⁶个精子）。（摘要截断于250字）
