Kliche S, Briese T, Henschen A H, Stitz L, Lipkin W I
Department of Neurology, University of California, Irvine 92717.
J Virol. 1994 Nov;68(11):6918-23. doi: 10.1128/JVI.68.11.6918-6923.1994.
Borna disease virus is a nonsegmented negative-strand RNA virus that causes neurologic disease in a wide variety of animal hosts. Here we describe identification and characterization of the first glycoprotein in this viral system. The 18-kDa glycoprotein, gp18, has been purified from infected rat brain. Isolation and microsequencing of this protein allowed identification of a 16.2-kDa open reading frame in the viral antigenome. Lectin binding and endoglycosidase sensitivity assays indicate that gp18 is an unusual N-linked glycoprotein.
博尔纳病病毒是一种不分节段的负链RNA病毒，可在多种动物宿主中引发神经疾病。在此，我们描述了该病毒系统中首个糖蛋白的鉴定与特性。18 kDa糖蛋白gp18已从受感染的大鼠脑中纯化出来。对该蛋白的分离和微量测序使得在病毒反基因组中鉴定出一个16.2 kDa的开放阅读框。凝集素结合和内切糖苷酶敏感性分析表明，gp18是一种不同寻常的N-连接糖蛋白。
