Briese T, Schneemann A, Lewis A J, Park Y S, Kim S, Ludwig H, Lipkin W I
Department of Neurology, University of California, Irvine 92717.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4362-6. doi: 10.1073/pnas.91.10.4362.
Borna disease virus is a neurotropic negative-strand RNA virus that infects a wide range of vertebrate hosts, causing disturbances in movement and behavior. We have cloned and sequenced the 8910-nucleotide viral genome by using RNA from Borna disease virus particles. The viral genome has complementary 3' and 5' termini and contains antisense information for five open reading frames. Homology to Filoviridae, Paramyxoviridae, and Rhabdoviridae is found in both cistronic and extracistronic regions. Northern analysis indicates that the virus transcribes mono- and polycistronic RNAs and uses termination/polyadenylylation signals reminiscent of those observed in other negative-strand RNA viruses. Borna disease virus is likely to represent a previously unrecognized genus, bornaviruses, or family, Bornaviridae, within the order Mononegavirales.
博尔纳病病毒是一种嗜神经性负链RNA病毒，可感染多种脊椎动物宿主，导致运动和行为紊乱。我们利用博尔纳病病毒颗粒中的RNA克隆并测序了8910个核苷酸的病毒基因组。该病毒基因组具有互补的3'和5'末端，并包含五个开放阅读框的反义信息。在顺反子和非顺反子区域均发现与丝状病毒科、副粘病毒科和弹状病毒科具有同源性。Northern分析表明，该病毒转录单顺反子和多顺反子RNA，并使用类似于在其他负链RNA病毒中观察到的终止/聚腺苷酸化信号。博尔纳病病毒可能代表单股负链RNA病毒目内一个以前未被认识的属——博尔纳病毒属，或科——博尔纳病毒科。
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