Schneider P A, Briese T, Zimmermann W, Ludwig H, Lipkin W I
Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
J Virol. 1994 Jan;68(1):63-8. doi: 10.1128/JVI.68.1.63-68.1994.
Coding and noncoding sequences were analyzed from field and experimental isolates of Borna disease virus. For a 24-kDa protein, maximum divergence was 1.5% at the predicted amino acid level and 3.1% at the nucleotide level. For a 40-kDa protein, maximum divergence was 1.1% at the predicted amino acid level and 4.1% at the nucleotide level. The highest variability in sequence (10%) was found in a 40-nucleotide stretch of genomic RNA between coding sequences for the 40- and 24-kDa proteins. The degree of sequence conservation in these isolates, passaged in various host species in vivo and in vitro over a period of 64 years, is unusual for negative-strand RNA viruses.
对博尔纳病病毒的野外分离株和实验分离株的编码及非编码序列进行了分析。对于一种24 kDa的蛋白质，预测氨基酸水平的最大差异为1.5%，核苷酸水平为3.1%。对于一种40 kDa的蛋白质，预测氨基酸水平的最大差异为1.1%，核苷酸水平为4.1%。在40 kDa和24 kDa蛋白质编码序列之间的一段40个核苷酸的基因组RNA中发现了最高的序列变异性（10%）。这些在64年的时间里在各种宿主物种体内和体外传代的分离株中的序列保守程度，对于负链RNA病毒来说是不寻常的。
