Thomas F P, Chalk C, Lalonde R, Robitaille Y, Jolicoeur P
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
J Virol. 1994 Nov;68(11):7099-107. doi: 10.1128/JVI.68.11.7099-7107.1994.
Patients infected with the human immunodeficiency virus type 1 (HIV-1) frequently develop central and peripheral nervous system complications, some of which may reflect the effect of the virus itself. In order to elucidate the pathogenic mechanisms of HIV in neurological disease in a small animal model, we generated transgenic mice expressing the entire HIV genome under control of the promoter for the human neurofilament NF-L gene. The transgene was predominantly expressed in anterior thalamic and spinal motor neurons. Animals developed a neurological syndrome characterized by hypoactivity and weakness and by axonal degeneration in peripheral nerves. These results provide evidence for a role of HIV in affecting both the central and peripheral nervous systems. This animal model may also facilitate the development of therapeutic agents against the human disease.
感染1型人类免疫缺陷病毒（HIV-1）的患者经常出现中枢和外周神经系统并发症，其中一些可能反映了病毒本身的作用。为了在小动物模型中阐明HIV在神经疾病中的致病机制，我们构建了在人神经丝NF-L基因启动子控制下表达完整HIV基因组的转基因小鼠。转基因主要在前丘脑和脊髓运动神经元中表达。动物出现了一种神经综合征，其特征为活动减少、虚弱以及外周神经轴突变性。这些结果为HIV在影响中枢和外周神经系统方面的作用提供了证据。这种动物模型也可能有助于开发针对人类疾病的治疗药物。
