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人巨细胞病毒IE2-580aa与pUL84在裂解感染细胞中的蛋白质-蛋白质相互作用。

Protein-protein interactions between human cytomegalovirus IE2-580aa and pUL84 in lytically infected cells.

作者信息

Spector D J, Tevethia M J

机构信息

Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.

出版信息

J Virol. 1994 Nov;68(11):7549-53. doi: 10.1128/JVI.68.11.7549-7553.1994.

DOI:10.1128/JVI.68.11.7549-7553.1994
PMID:7933141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237200/
Abstract

The human cytomegalovirus immediate-early protein IE2-580aa (ppUL122a) activates transcription of viral and cellular genes and represses its own transcription through sequence-specific binding to the major immediate-early promoter. In lytically infected cells, IE2-580aa interacts with a 75-kDa viral protein (p75), an early protein that is also synthesized at late times after infection. Here we show that p75 is the product of the UL84 gene. Its association with IE2-580aa in infected cells suggests that pUL84 is involved in transcription control.

摘要

人巨细胞病毒立即早期蛋白IE2 - 580aa（ppUL122a）通过与主要立即早期启动子的序列特异性结合来激活病毒和细胞基因的转录，并抑制其自身的转录。在裂解感染的细胞中，IE2 - 580aa与一种75 kDa的病毒蛋白（p75）相互作用，p75是一种早期蛋白，在感染后期也会合成。在这里我们表明p75是UL84基因的产物。它在感染细胞中与IE2 - 580aa的关联表明pUL84参与转录调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/226dd42a19ef/jvirol00020-0739-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/fbad5f583a0a/jvirol00020-0739-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/f15f3ccbc55c/jvirol00020-0739-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/226dd42a19ef/jvirol00020-0739-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/fbad5f583a0a/jvirol00020-0739-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/f15f3ccbc55c/jvirol00020-0739-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c893/237200/226dd42a19ef/jvirol00020-0739-c.jpg

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本文引用的文献

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2
Direct interaction of the human cytomegalovirus IE86 protein with the cis repression signal does not preclude TBP from binding to the TATA box.人类巨细胞病毒IE86蛋白与顺式抑制信号的直接相互作用并不妨碍TBP与TATA盒结合。
J Virol. 1993 Sep;67(9):5595-604. doi: 10.1128/JVI.67.9.5595-5604.1993.
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Human cytomegalovirus immediate-early gene 2 protein interacts with itself and with several novel cellular proteins.
Human Cytomegalovirus Primary Infection and Reactivation: Insights From Virion-Carried Molecules.
人巨细胞病毒原发性感染与再激活：来自病毒体携带分子的见解
Front Microbiol. 2020 Jul 14;11:1511. doi: 10.3389/fmicb.2020.01511. eCollection 2020.
4
Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli.人巨细胞病毒UL84的动态且依赖核仁素的定位至病毒复制区室和核仁的周边。
J Virol. 2014 Oct;88(20):11738-47. doi: 10.1128/JVI.01889-14. Epub 2014 Jul 30.
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