Chhabra S K, Rao A R
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
J Ethnopharmacol. 1994 May;42(3):169-77. doi: 10.1016/0378-8741(94)90082-5.
The present study examines the possible transfer of the active principle(s) of mace (aril of the plant Myristica fragrans) through the transmammary route and its ability to modulate hepatic xenobiotic metabolizing enzymes in the F1 progeny of mice. An aqueous suspension of mace at the dose levels of 0.025 or 0.1 g/animal/day was administered by oral gavage to dams from day 1 of lactation and continued daily for 14 or 21 days. Dams receiving mace treatment and their F1 pups showed significantly elevated hepatic sulfhydryl content, glutathione S-transferase and glutathione reductase activities and cytochrome b5 content. Hepatic cytochrome P450 content decreased in dams (P < 0.05) receiving the lower mace dose for 21 days and the F1 pups (P < 0.001), but increased in dams receiving the higher dose for both time periods (P < 0.001) and the lower dose for 14 days (P < 0.05). Only the 14-day-old pups of dams receiving either mace dose showed significantly elevated (P < 0.001) levels of hepatic glutathione peroxidase.
本研究检测了肉豆蔻（肉豆蔻科植物肉豆蔻的假种皮）活性成分通过乳腺途径的可能传递情况及其对小鼠F1代肝脏异生物质代谢酶的调节能力。从哺乳期第1天开始，通过灌胃给母鼠每日投喂剂量为0.025或0.1 g/动物/天的肉豆蔻水悬浮液，持续14天或21天。接受肉豆蔻处理的母鼠及其F1代幼崽的肝脏巯基含量、谷胱甘肽S - 转移酶和谷胱甘肽还原酶活性以及细胞色素b5含量均显著升高。接受较低剂量肉豆蔻处理21天的母鼠（P < 0.05）和F1代幼崽（P < 0.001）肝脏细胞色素P450含量降低，但在两个时间段接受较高剂量的母鼠（P < 0.001）和接受较低剂量14天的母鼠（P < 0.05）中细胞色素P450含量增加。仅接受任一肉豆蔻剂量的母鼠所产14日龄幼崽的肝脏谷胱甘肽过氧化物酶水平显著升高（P < 0.001）。
