Chhabra S K, Hashim S, Rao A R
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
J Appl Toxicol. 1993 Nov-Dec;13(6):411-6. doi: 10.1002/jat.2550130607.
The present study examines the transmammary modulation of the glutathione system of enzymes in the F1 generation of mouse pups postnatally exposed to malathion. Lactating Swiss albino mice received either 30 or 100 mg malathion kg-1 body wt. (98% pure) for 14 or 21 days postpartum. The acid-soluble sulphydryl content was significantly increased (P < 0.001) in the liver of 14-day-old pups of dams that had received the higher malathion dose. A similar significant increase was seen in the 21-day-old male pups of dams that had received 30 mg (P < 0.05) or 100 mg (P < 0.01) malathion kg-1 body wt. Dams showed an enhanced hepatic glutathione S-transferase activity following treatment with 100 mg malathion kg-1 body wt. for 14 days (P < 0.02) and 21 days (P < 0.001). Pups of either age groups also showed enhanced hepatic glutathione S-transferase activity (P < 0.001). A significant enhancement in glutathione reductase activity was observed with malathion treatment in livers of dams and pups (P < 0.001). However, dams that had received 30 mg malathion kg-1 body wt. daily for 21 days or 100 mg malathion kg-1 body wt. for either 14 or 21 days showed significantly reduced hepatic glutathione peroxidase activity (P < 0.01, P < 0.001). A significant decrease in glutathione peroxidase activity was also observed in the liver of the 21-day-old male (P < 0.01) and female (P < 0.02) pups of dams that were treated with the higher dose of malathion.
本研究检测了产后暴露于马拉硫磷的F1代幼鼠乳腺中谷胱甘肽酶系统的变化。泌乳期的瑞士白化小鼠在产后14天或21天接受30或100 mg/kg体重（98%纯品）的马拉硫磷。接受较高马拉硫磷剂量的母鼠所产14日龄幼鼠肝脏中酸溶性巯基含量显著增加（P < 0.001）。接受30 mg/kg体重（P < 0.05）或100 mg/kg体重（P < 0.01）马拉硫磷的母鼠所产21日龄雄性幼鼠也出现类似的显著增加。母鼠在接受100 mg/kg体重马拉硫磷处理14天（P < 0.02）和21天（P < 0.001）后，肝脏谷胱甘肽S-转移酶活性增强。两个年龄组的幼鼠肝脏谷胱甘肽S-转移酶活性也增强（P < 0.001）。马拉硫磷处理后，母鼠和幼鼠肝脏中的谷胱甘肽还原酶活性显著增强（P < 0.001）。然而，每天接受30 mg/kg体重马拉硫磷处理21天或接受100 mg/kg体重马拉硫磷处理14天或21天的母鼠肝脏谷胱甘肽过氧化物酶活性显著降低（P < 0.01，P < 0.001）。接受较高剂量马拉硫磷处理的母鼠所产21日龄雄性（P < 0.01）和雌性（P < 0.02）幼鼠肝脏中的谷胱甘肽过氧化物酶活性也显著降低。
