Chhabra S K, Rao A R
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University (J.N.U.), New Delhi, India.
Food Chem Toxicol. 1994 Jun;32(6):527-31. doi: 10.1016/0278-6915(94)90109-0.
In an investigation of the modulation of certain neonatal xenobiotic-metabolizing enzymes in liver of mouse pups postnatally exposed to selenium through the transmammary route, sodium selenite was administered in drinking water to lactating dams at the dose levels of 1 or 5 ppm from day 1 of lactation and continued daily for 14 or 21 days. The higher dose of selenium was found to increase the hepatic acid-soluble sulfhydryl content significantly after 21 days of treatment in dams, their pups (P < 0.01) and in the 14-day-old male pups (P < 0.05). Cytochrome b5 content decreased in the livers of dams that received 5 ppm selenium (P < 0.01) and in the F1 pups (P < 0.01) translactationally exposed to selenium for 14 days. Cytochrome P-450 content decreased in dams and pups exposed to 5 ppm selenium for 14 days and either dose for 21 days (P < 0.01). Hepatic glutathione S-transferase decreased in the dam that had received 5 ppm selenium for 14 days (P < 0.05) and in the 14-day-old pups (P < 0.01). Glutathione reductase and glutathione peroxidase activities decreased in both dams and pups (P < 0.01). The overall suppression of neonatal hepatic detoxification enzymes demonstrates that selenium may have far-reaching consequences on neonatal growth, development and drug pharmacokinetics.
在一项关于通过母乳途径产后暴露于硒的幼鼠肝脏中某些新生儿异生物质代谢酶调节的研究中，从哺乳期第1天起，以1或5 ppm的剂量水平将亚硒酸钠添加到饮用水中给予哺乳母鼠，并持续每日给药14或21天。在母鼠、它们的幼崽（P < 0.01）以及14日龄雄性幼崽（P < 0.05）中，较高剂量的硒在治疗21天后被发现可显著增加肝脏酸溶性巯基含量。接受5 ppm硒的母鼠（P < 0.01）以及经母乳暴露于硒14天的F1幼崽（P < 0.01）肝脏中的细胞色素b5含量降低。暴露于5 ppm硒14天以及任一剂量21天的母鼠和幼崽中细胞色素P - 450含量降低（P < 0.01）。接受5 ppm硒14天的母鼠（P < 0.05）以及14日龄幼崽（P < 0.01）肝脏中的谷胱甘肽S - 转移酶降低。母鼠和幼崽中谷胱甘肽还原酶和谷胱甘肽过氧化物酶活性均降低（P < 0.01）。新生儿肝脏解毒酶的总体抑制表明，硒可能对新生儿生长、发育和药物药代动力学产生深远影响。
