• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Ets-1在B细胞中激活DRA启动子。

Ets-1 activates the DRA promoter in B cells.

作者信息

Jabrane-Ferrat N, Peterlin B M

机构信息

Howard Hughes Medical Institute, University of California at San Francisco, 94143-0724.

出版信息

Mol Cell Biol. 1994 Nov;14(11):7314-21. doi: 10.1128/mcb.14.11.7314-7321.1994.

DOI:10.1128/mcb.14.11.7314-7321.1994
PMID:7935445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359266/
Abstract

The X box in promoters of class II major histocompatibility complex genes plays a crucial role in the B-cell-specific and gamma interferon-inducible expression of these genes. The sequence TTCC is located in the pyrimidine tract which extends 5' to and partially overlaps the X box of the DRA promoter. This sequence resembles the core binding site for the Ets family of DNA-binding proteins. In this study, we demonstrate that mutations within the pyrimidine tract which change the TTCC motif, but do not affect the binding of regulatory factor X to the X box, decrease the activity of the DRA promoter in B cells. Furthermore, using electrophoretic mobility shift assays and cotransfection experiments, we demonstrate that Ets-1, but not Ets-2 or PU.1, functionally interacts with the pyrimidine tract and activates the DRA promoter.

摘要

II类主要组织相容性复合体基因启动子中的X盒在这些基因的B细胞特异性和γ干扰素诱导性表达中起关键作用。序列TTCC位于嘧啶序列中，该嘧啶序列从DRA启动子的X盒5'端延伸并部分重叠。该序列类似于DNA结合蛋白Ets家族的核心结合位点。在本研究中，我们证明嘧啶序列内改变TTCC基序但不影响调节因子X与X盒结合的突变会降低DRA启动子在B细胞中的活性。此外，通过电泳迁移率变动分析和共转染实验，我们证明Ets-1而非Ets-2或PU.1在功能上与嘧啶序列相互作用并激活DRA启动子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/bcc645eedc60/molcellb00011-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/0977df5c0b1f/molcellb00011-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/fda68ef119ce/molcellb00011-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/2a9ae0007cdd/molcellb00011-0291-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/7a7816041b0f/molcellb00011-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/bcc645eedc60/molcellb00011-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/0977df5c0b1f/molcellb00011-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/fda68ef119ce/molcellb00011-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/2a9ae0007cdd/molcellb00011-0291-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/7a7816041b0f/molcellb00011-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8ca/359266/bcc645eedc60/molcellb00011-0293-a.jpg

相似文献

1
Ets-1 activates the DRA promoter in B cells.Ets-1在B细胞中激活DRA启动子。
Mol Cell Biol. 1994 Nov;14(11):7314-21. doi: 10.1128/mcb.14.11.7314-7321.1994.
2
Role of the X2 box in activated transcription from the DRA promoter in B cells.
Immunogenetics. 1997;46(4):318-25. doi: 10.1007/s002510050278.
3
In vivo footprint analysis of the HLA-DRA gene promoter: cell-specific interaction at the octamer site and up-regulation of X box binding by interferon gamma.HLA - DRA基因启动子的体内足迹分析：八聚体位点的细胞特异性相互作用及干扰素γ对X盒结合的上调作用
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7601-5. doi: 10.1073/pnas.89.16.7601.
4
Pax-5 (BSAP) recruits Ets proto-oncogene family proteins to form functional ternary complexes on a B-cell-specific promoter.配对盒基因5（BSAP）募集Ets原癌基因家族蛋白，以在B细胞特异性启动子上形成功能性三元复合物。
Genes Dev. 1996 Sep 1;10(17):2198-211. doi: 10.1101/gad.10.17.2198.
5
Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.将II类主要组织相容性复合体突变细胞系克隆-13中缺陷转录因子的相互作用位点定位到不同的X2盒。
J Biol Chem. 1995 Mar 17;270(11):6396-402. doi: 10.1074/jbc.270.11.6396.
6
Functional roles of the transcription factor Oct-2A and the high mobility group protein I/Y in HLA-DRA gene expression.转录因子Oct-2A和高迁移率族蛋白I/Y在HLA-DRA基因表达中的功能作用。
J Exp Med. 1995 Aug 1;182(2):487-500. doi: 10.1084/jem.182.2.487.
7
The function of the octamer-binding site in the DRA promoter.DRA启动子中八聚体结合位点的功能。
Immunogenetics. 1996;43(1-2):20-6. doi: 10.1007/BF00186600.
8
Two B cell factors bind the HLA-DRA X box region and recognize different subsets of HLA class II promoters.两种B细胞因子结合HLA - DRA X盒区域并识别HLA II类启动子的不同亚群。
Nucleic Acids Res. 1991 Nov 25;19(22):6269-76. doi: 10.1093/nar/19.22.6269.
9
Tumor necrosis factor alpha response elements in the HLA-DRA promoter: identification of a tumor necrosis factor alpha-induced DNA-protein complex in astrocytes.HLA - DRA启动子中的肿瘤坏死因子α反应元件：星形胶质细胞中肿瘤坏死因子α诱导的DNA - 蛋白质复合物的鉴定
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11518-22. doi: 10.1073/pnas.89.23.11518.
10
Dual regulation of ets-activated gene expression by SP1.SP1对ets激活基因表达的双重调控。
Gene. 2003 Mar 27;307:87-97. doi: 10.1016/s0378-1119(03)00445-1.

引用本文的文献

1
Genome-Wide Identification of Target Genes for the Key B Cell Transcription Factor .关键B细胞转录因子靶基因的全基因组鉴定
Front Immunol. 2017 Apr 7;8:383. doi: 10.3389/fimmu.2017.00383. eCollection 2017.
2
Function and regulation of class II transactivator in the immune system.II类反式激活因子在免疫系统中的功能与调控
Immunol Res. 2002;25(2):131-42. doi: 10.1385/IR:25:2:131.
3
Self-association of CIITA and its transactivation potential.CIITA的自我缔合及其反式激活潜能。

本文引用的文献

1
The biochemistry and cell biology of antigen processing and presentation.抗原加工与呈递的生物化学和细胞生物学
Annu Rev Immunol. 1993;11:403-50. doi: 10.1146/annurev.iy.11.040193.002155.
2
The molecular descent of the major histocompatibility complex.主要组织相容性复合体的分子谱系
Annu Rev Immunol. 1993;11:269-95. doi: 10.1146/annurev.iy.11.040193.001413.
3
Synergistic activation of the HTLV1 LTR Ets-responsive region by transcription factors Ets1 and Sp1.转录因子Ets1和Sp1对HTLV1长末端重复序列Ets反应区域的协同激活作用。
Mol Cell Biol. 2001 Aug;21(15):4919-28. doi: 10.1128/MCB.21.15.4919-4928.2001.
4
Mutations in the bare lymphocyte syndrome define critical steps in the assembly of the regulatory factor X complex.裸淋巴细胞综合征中的突变确定了调节因子X复合体组装中的关键步骤。
Mol Cell Biol. 2000 Jun;20(12):4455-61. doi: 10.1128/MCB.20.12.4455-4461.2000.
5
The class II trans-activator CIITA interacts with the TBP-associated factor TAFII32.II类反式激活因子CIITA与TBP相关因子TAFII32相互作用。
Nucleic Acids Res. 1997 Jun 15;25(12):2522-8. doi: 10.1093/nar/25.12.2522.
6
Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.主要组织相容性复合体II类启动子的复杂结构：重复基序和保守的蛋白质-蛋白质相互作用。
Mol Cell Biol. 1996 Sep;16(9):4683-90. doi: 10.1128/MCB.16.9.4683.
7
Binding and cooperative interactions between two B cell-specific transcriptional coactivators.两种B细胞特异性转录共激活因子之间的结合与协同相互作用。
J Exp Med. 1996 Jun 1;183(6):2517-21. doi: 10.1084/jem.183.6.2517.
EMBO J. 1993 Mar;12(3):1169-78. doi: 10.1002/j.1460-2075.1993.tb05758.x.
4
Regulation of MHC gene expression.MHC基因表达的调控。
Curr Opin Immunol. 1993 Feb;5(1):8-16. doi: 10.1016/0952-7915(93)90074-3.
5
Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression.Ets蛋白：调节免疫球蛋白重链基因表达的新因子。
Mol Cell Biol. 1993 Nov;13(11):7163-9. doi: 10.1128/mcb.13.11.7163-7169.1993.
6
The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain.血清反应因子辅助蛋白Elk-1含有一个受生长因子调控的转录激活结构域。
Cell. 1993 Apr 23;73(2):381-93. doi: 10.1016/0092-8674(93)90237-k.
7
Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.ETS 结构域蛋白对淋巴特异性免疫球蛋白 μ 重链基因增强子的调控
Science. 1993 Jul 2;261(5117):82-6. doi: 10.1126/science.8316859.
8
The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.Ets1转录因子在小鼠胚胎发育过程中广泛表达，并与参与器官形成等形态发生过程的中胚层细胞相关。
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7588-92. doi: 10.1073/pnas.90.16.7588.
9
Trans-acting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells.人嗜T淋巴细胞病毒长末端重复序列在受感染细胞中的反式作用转录激活
Science. 1984 Jul 27;225(4660):381-5. doi: 10.1126/science.6330891.
10
Identification in chickens of an evolutionarily conserved cellular ets-2 gene (c-ets-2) encoding nuclear proteins related to the products of the c-ets proto-oncogene.在鸡中鉴定出一种进化上保守的细胞ets - 2基因（c - ets - 2），该基因编码与c - ets原癌基因产物相关的核蛋白。
EMBO J. 1988 Mar;7(3):697-705. doi: 10.1002/j.1460-2075.1988.tb02865.x.