Lipperheide V, Andraka L, Pontón J, Quindós G
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
Mycoses. 1993 Nov-Dec;36(11-12):417-20. doi: 10.1111/j.1439-0507.1993.tb00731.x.
Albicans IDR is a ready-to-use medium containing a hexosaminic substrate that allows rapid and specific identification of Candida albicans. In this study, we have evaluated its performance using 330 pure cultures of yeast strains previously identified by conventional mycological methods. The test correctly identified all the Candida albicans strains tested (186 isolates), but there were 28 false-positive reactions after 48 h of culture incubation. The false-positive isolates corresponded to the species Candida tropicalis (13 strains), Trichosporon beigelii (12 strains), Cryptococcus neoformans (one strain), Candida lusitaniae (one strain) and Candida membranaefaciens (one strain). The sensitivity of the method was 100% and the specificity was 90.3% at 24 h and 86.6% at 48 h of incubation. Albicans IDR plate is a new, easy-to-perform and inexpensive method for C. albicans identification, and it could be used for direct isolation and identification of this species from clinical samples.
白色念珠菌IDR是一种即用型培养基，含有一种己糖胺底物，可快速、特异性地鉴定白色念珠菌。在本研究中，我们使用330株先前通过传统真菌学方法鉴定的酵母菌株纯培养物评估了其性能。该测试正确鉴定了所有测试的白色念珠菌菌株（186株分离株），但培养孵育48小时后有28例假阳性反应。假阳性分离株对应于热带念珠菌（13株）、白吉利丝孢酵母（12株）、新型隐球菌（1株）、葡萄牙念珠菌（1株）和膜醭念珠菌（1株）。该方法在孵育24小时时的灵敏度为100%，特异性为90.3%，在孵育48小时时的特异性为86.6%。白色念珠菌IDR平板是一种用于鉴定白色念珠菌的新的、易于操作且廉价的方法，可用于从临床样本中直接分离和鉴定该菌种。
