三种用于直接鉴定和区分临床标本中念珠菌属菌种的商业培养基的比较
Comparison of three commercial media for direct identification and discrimination of Candida species in clinical specimens.
作者信息
Freydière A M, Buchaille L, Gille Y
机构信息
Laboratoire de Bactériologie, Hôpital de l'Antiquaille, Lyon, France.
出版信息
Eur J Clin Microbiol Infect Dis. 1997 Jun;16(6):464-7. doi: 10.1007/BF02471913.
One hundred and ninety-two clinical specimens were used to compare the three chromogenic media Albicans ID, Candiselect, and CHROMagar Candida to a standard method using a Sabouraud-chloramphenicol agar medium and standard methods for identification of yeasts. The detection rates were 83.79, 83.24, 86.59 and 84.91% respectively. For the chromogenic media, the rates of direct identification (growth plus specific pigmentation) for Candida albicans were 56.50, 37.68 and 11.59% after 24 hours' incubation and 92.75, 91.30 and 88.57% after 72 hours' incubation respectively, with 100% specificity. Furthermore, CHROMagar Candida identified the five Candida tropicalis and the two Candida krusei strains detected after 48 hours' incubation.
使用192份临床标本，将三种显色培养基（白色念珠菌ID、念珠菌选择培养基和CHROMagar念珠菌培养基）与使用沙氏氯霉素琼脂培养基的标准方法以及酵母鉴定的标准方法进行比较。检测率分别为83.79%、83.24%、86.59%和84.91%。对于显色培养基，白色念珠菌在培养24小时后的直接鉴定率（生长加特定色素沉着）分别为56.50%、37.68%和11.59%，培养72小时后分别为92.75%、91.30%和88.57%，特异性为100%。此外，CHROMagar念珠菌培养基在培养48小时后鉴定出了检测到的5株热带念珠菌和2株克鲁斯念珠菌菌株。
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