Mutoh S, Kumada K, Kume K, Suzuki J, Yokota T, Suzuki H
Department of Pediatrics, Fukushima Medical College, Japan.
Nephron. 1994;67(3):340-5. doi: 10.1159/000187990.
We describe a method for detecting virus-specific RNA sequences using a nonradioactive enzyme system, and analyze coxsackie B4 virus (cox.B4) RNA sequences in infected mouse kidney with pathological changes. Diffuse mesangial proliferation was observed transiently 24 h after inoculation of cox.B4 virus in glomeruli. OCT-treated thin sections of the same tissue were hybridized in situ using a digoxigenin-labeled oligonucleotide probe. The strongest signal was observed in the mesangial sample. These findings suggest that the transient proliferative mesangial changes were caused by direct injury following virus infection without immune complex.
我们描述了一种使用非放射性酶系统检测病毒特异性RNA序列的方法，并分析了感染柯萨奇B4病毒（cox.B4）且有病理变化的小鼠肾脏中的RNA序列。接种cox.B4病毒24小时后，在肾小球中短暂观察到弥漫性系膜增生。使用地高辛标记的寡核苷酸探针，对同一组织经OCT处理的薄切片进行原位杂交。在系膜样本中观察到最强信号。这些发现表明，短暂的系膜增生性变化是由病毒感染后的直接损伤引起的，而非免疫复合物所致。
