Vella C, Easton A J, Eglin R P, Brown C L, Perry L
Department of Immunology, Royal London Hospital Medical College, England.
J Med Virol. 1991 Sep;35(1):46-9. doi: 10.1002/jmv.1890350110.
The pathology of Coxsackie virus B4 (CVB4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cDNA probe derived from CVB4. During the acute phase of infection virus RNA sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of CVB4. A positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. The cellular distribution of virus RNA sequences corresponded well with the histological findings in most tissues. Possible causes for failure of hybridisation in some infected pancreases are discussed in conjunction with potential application of the technique in human pancreas biopsy samples.
利用源自柯萨奇病毒B4（CVB4）的生物素化cDNA探针，通过原位杂交研究了CVB4感染小鼠模型的病理学。在感染急性期，60%感染CVB4胰腺嗜性变体的小鼠外分泌胰腺中检测到病毒RNA序列。在一些动物的其他器官中也观察到阳性杂交信号，包括1只小鼠感染28天后的心脏和肝脏。病毒RNA序列的细胞分布与大多数组织的组织学发现非常吻合。结合该技术在人类胰腺活检样本中的潜在应用，讨论了一些受感染胰腺杂交失败的可能原因。
