Hilton D A, Day C, Pringle J H, Fletcher A, Chambers S
Department of Pathology, Leicester Royal Infirmary, UK.
J Virol Methods. 1992 Nov;40(2):155-62. doi: 10.1016/0166-0934(92)90064-k.
A simple method for the demonstration of Coxsackie virus RNA by in situ hybridization is described. Oligonucleotides complimentary to conserved sequences of Coxsackie B genome were synthesised and labelled with digoxigenin using commercially available reagents. In addition to detecting all five Coxsackie B strains examined, six strains of Coxsackie A were also demonstrated by these probes. Using one of the oligonucleotides separately it was possible to distinguish Coxsackie A strains from the strains of Coxsackie B virus examined. This study demonstrates the presence of viral RNA in mice tissues showing morphological evidence of damage, confirming the suspected tropisms of these viruses. The method described is directly applicable to the study of the presence of these viruses in human tissue from diseases where a viral aetiology is suspected.
本文描述了一种通过原位杂交检测柯萨奇病毒RNA的简单方法。使用市售试剂合成了与柯萨奇B基因组保守序列互补的寡核苷酸，并用地高辛标记。这些探针除了能检测所检测的所有五种柯萨奇B毒株外，还能检测六种柯萨奇A毒株。单独使用其中一种寡核苷酸可以区分柯萨奇A毒株和所检测的柯萨奇B病毒毒株。本研究证明了在显示损伤形态学证据的小鼠组织中存在病毒RNA，证实了这些病毒的疑似嗜性。所描述的方法可直接应用于研究疑似病毒病因的疾病患者人体组织中这些病毒的存在情况。
