粗糙脉孢菌（1,3）β-葡聚糖合酶活性及细胞壁形成所需基因的克隆与特性分析
Cloning and characterization of a Neurospora crassa gene required for (1,3) beta-glucan synthase activity and cell wall formation.
作者信息
Enderlin C S, Selitrennikoff C P
机构信息
Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262.
出版信息
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9500-4. doi: 10.1073/pnas.91.20.9500.
Abstract
The glucan synthase 1 gene (gs-1) is required for (1,3) beta-glucan synthase activity [E.C. 2.4.1.34; UDP glucose:1,3-beta-D-glucan 3-beta-D-glucosyltransferase] and for cell wall formation. The gs-1 gene was cloned by functional complementation of the cell-wall-less defect of the (1,3) beta-glucan synthase-deficient mutant, TM1, by using a genomic Neurospora crassa cosmid library. A 2568-nucleotide gs-1 cDNA sequence revealed a 532-amino acid open reading frame encoding a polypeptide of 59 kDa. The predicted gs-1 gene product has no obvious signal peptide cleavage sites or transmembrane domains. A gs-1 null mutant is defective for cell wall formation and (1,3) beta-glucan synthase activity. The predicted GS-1 protein is weakly homologous to a putative Saccharomyces cerevisiae transcriptional regulatory protein.
摘要
葡聚糖合酶1基因（gs-1）对于（1,3）β-葡聚糖合酶活性[E.C. 2.4.1.34；UDP葡萄糖：1,3-β-D-葡聚糖3-β-D-葡糖基转移酶]以及细胞壁形成是必需的。通过使用粗糙脉孢菌基因组黏粒文库对（1,3）β-葡聚糖合酶缺陷型突变体TM1的无细胞壁缺陷进行功能互补，克隆了gs-1基因。一个2568个核苷酸的gs-1 cDNA序列揭示了一个532个氨基酸的开放阅读框，编码一个59 kDa的多肽。预测的gs-1基因产物没有明显的信号肽切割位点或跨膜结构域。一个gs-1基因缺失突变体在细胞壁形成和（1,3）β-葡聚糖合酶活性方面存在缺陷。预测的GS-1蛋白与一种推定的酿酒酵母转录调节蛋白有弱同源性。
相似文献
1
Cloning and characterization of a Neurospora crassa gene required for (1,3) beta-glucan synthase activity and cell wall formation.粗糙脉孢菌（1,3）β-葡聚糖合酶活性及细胞壁形成所需基因的克隆与特性分析
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9500-4. doi: 10.1073/pnas.91.20.9500.
2
Inhibition of Neurospora crassa growth by a glucan synthase-1 antisense construct.葡聚糖合酶-1反义构建体对粗糙脉孢菌生长的抑制作用。
Curr Microbiol. 1997 May;34(5):303-8. doi: 10.1007/s002849900186.
3
cps1+, a Schizosaccharomyces pombe gene homolog of Saccharomyces cerevisiae FKS genes whose mutation confers hypersensitivity to cyclosporin A and papulacandin B.cps1+，一种粟酒裂殖酵母基因，是酿酒酵母FKS基因的同源物，其突变会导致对环孢菌素A和丘疹霉素B过敏。
J Bacteriol. 1997 Dec;179(24):7653-62. doi: 10.1128/jb.179.24.7653-7662.1997.
4
Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in beta-1,3-glucan synthesis.白色念珠菌酿酒酵母GSC1/FKS1同源物的克隆及其在β-1,3-葡聚糖合成中的作用。
J Bacteriol. 1997 Jul;179(13):4096-105. doi: 10.1128/jb.179.13.4096-4105.1997.
5
Isolation of a gene involved in 1,3-beta-glucan synthesis in Aspergillus nidulans and purification of the corresponding protein.构巢曲霉中参与1,3-β-葡聚糖合成的基因的分离及相应蛋白质的纯化。
J Bacteriol. 1996 Aug;178(15):4381-91. doi: 10.1128/jb.178.15.4381-4391.1996.
6
Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.芽殖酵母和裂殖酵母中对丘疹霉素B的抗性：酿酒酵母中参与（1,3）β-D-葡聚糖合成的一个基因的分离与特性分析
J Bacteriol. 1995 Oct;177(20):5732-9. doi: 10.1128/jb.177.20.5732-5739.1995.
7
Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro.酵母β-葡聚糖合成：KRE6编码一种预测的II型膜蛋白，该蛋白在体内葡聚糖合成以及体外葡聚糖合酶活性方面是必需的。
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11295-9. doi: 10.1073/pnas.88.24.11295.
8
The Schizosaccharomyces pombe cwg2+ gene codes for the beta subunit of a geranylgeranyltransferase type I required for beta-glucan synthesis.粟酒裂殖酵母cwg2+基因编码β-葡聚糖合成所需的I型香叶基香叶基转移酶的β亚基。
EMBO J. 1993 Dec 15;12(13):5245-54. doi: 10.1002/j.1460-2075.1993.tb06220.x.
9
Cloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis.参与（1,3）-β-葡聚糖合成的酵母基因KNR4的克隆与特性分析
Mol Cell Biol. 1994 Feb;14(2):1017-25. doi: 10.1128/mcb.14.2.1017-1025.1994.
10
Cloning of the RHO1 gene from Candida albicans and its regulation of beta-1,3-glucan synthesis.白色念珠菌RHO1基因的克隆及其对β-1,3-葡聚糖合成的调控。
J Bacteriol. 1997 Dec;179(24):7734-41. doi: 10.1128/jb.179.24.7734-7741.1997.
引用本文的文献
1
The GPI-Anchored GH76 Protein Dfg5 Affects Hyphal Morphology and Osmoregulation in the Mycoparasite and Is Interconnected With MAPK Signaling.糖基磷脂酰肌醇锚定的GH76蛋白Dfg5影响真菌寄生菌的菌丝形态和渗透调节，并与丝裂原活化蛋白激酶信号传导相互关联。
Front Microbiol. 2021 Feb 10;12:601113. doi: 10.3389/fmicb.2021.601113. eCollection 2021.
2
Off the wall: The rhyme and reason of hyphal morphogenesis.超乎寻常：菌丝形态发生的规律与缘由
Cell Surf. 2019 Mar 8;5:100020. doi: 10.1016/j.tcsw.2019.100020. eCollection 2019 Dec.
3
A conserved fungal hub protein involved in adhesion and drug resistance in the human pathogen .一种参与人类病原体黏附和耐药性的保守真菌枢纽蛋白。
Cell Surf. 2018 Oct 29;4:10-19. doi: 10.1016/j.tcsw.2018.10.002. eCollection 2018 Dec.
4
The Genetics and Biochemistry of Cell Wall Structure and Synthesis in , a Model Filamentous Fungus.丝状真菌模式生物中的细胞壁结构与合成的遗传学和生物化学
Front Microbiol. 2019 Oct 10;10:2294. doi: 10.3389/fmicb.2019.02294. eCollection 2019.
5
Hyphal ontogeny in : a model organism for all seasons.《中的菌丝个体发育：适用于所有季节的模式生物》 （此标题翻译不太完整准确，因为原文表述不太清晰完整，推测这可能是一篇文章标题之类的内容，仅按字面翻译了）
F1000Res. 2016 Nov 30;5:2801. doi: 10.12688/f1000research.9679.1. eCollection 2016.
6
Circadian activation of the mitogen-activated protein kinase MAK-1 facilitates rhythms in clock-controlled genes in Neurospora crassa.有丝分裂原活化蛋白激酶MAK-1的昼夜激活促进了粗糙脉孢菌中生物钟控制基因的节律。
Eukaryot Cell. 2013 Jan;12(1):59-69. doi: 10.1128/EC.00207-12. Epub 2012 Nov 2.
7
Mutational analysis of the glycosylphosphatidylinositol (GPI) anchor pathway demonstrates that GPI-anchored proteins are required for cell wall biogenesis and normal hyphal growth in Neurospora crassa.糖基磷脂酰肌醇（GPI）锚定途径的突变分析表明，GPI锚定蛋白是粗糙脉孢菌细胞壁生物合成和正常菌丝生长所必需的。
Eukaryot Cell. 2006 Mar;5(3):587-600. doi: 10.1128/EC.5.3.587-600.2006.
8
The genetic basis of cellular morphogenesis in the filamentous fungus Neurospora crassa.丝状真菌粗糙脉孢菌细胞形态发生的遗传基础。
Mol Biol Cell. 2003 Nov;14(11):4352-64. doi: 10.1091/mbc.e02-07-0433. Epub 2003 Sep 5.
9
Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.芽殖酵母和裂殖酵母中对丘疹霉素B的抗性：酿酒酵母中参与（1,3）β-D-葡聚糖合成的一个基因的分离与特性分析
J Bacteriol. 1995 Oct;177(20):5732-9. doi: 10.1128/jb.177.20.5732-5739.1995.
10
Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae.酿酒酵母细胞完整性和形态发生的分子基础。
Microbiol Rev. 1995 Sep;59(3):345-86. doi: 10.1128/mr.59.3.345-386.1995.
本文引用的文献
1
Efficient cloning of genes of Neurospora crassa.高效克隆粗糙脉孢菌基因。
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4869-73. doi: 10.1073/pnas.83.13.4869.
2
Yeast calmodulin and a conserved nuclear protein participate in the in vivo binding of a matrix association region.酵母钙调蛋白和一种保守的核蛋白参与了基质附着区域的体内结合。
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5623-7. doi: 10.1073/pnas.90.12.5623.
3
A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis.酿酒酵母中杀伤毒素抗性的突变分析鉴定出参与细胞壁（1→6）-β-葡聚糖合成的新基因。
Genetics. 1993 Apr;133(4):837-49. doi: 10.1093/genetics/133.4.837.
4
GenBank.基因银行
Nucleic Acids Res. 1993 Jul 1;21(13):2963-5. doi: 10.1093/nar/21.13.2963.
5
Cloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis.参与（1,3）-β-葡聚糖合成的酵母基因KNR4的克隆与特性分析
Mol Cell Biol. 1994 Feb;14(2):1017-25. doi: 10.1128/mcb.14.2.1017-1025.1994.
6
The Schizosaccharomyces pombe cwg2+ gene codes for the beta subunit of a geranylgeranyltransferase type I required for beta-glucan synthesis.粟酒裂殖酵母cwg2+基因编码β-葡聚糖合成所需的I型香叶基香叶基转移酶的β亚基。
EMBO J. 1993 Dec 15;12(13):5245-54. doi: 10.1002/j.1460-2075.1993.tb06220.x.
7
Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis.酿酒酵母基因的克隆，该基因的过表达可克服HM-1杀伤毒素的作用，HM-1杀伤毒素会抑制β-葡聚糖的合成。
J Bacteriol. 1994 Mar;176(5):1488-99. doi: 10.1128/jb.176.5.1488-1499.1994.
8
One-step gene disruption in yeast.酵母中的一步基因破坏
Methods Enzymol. 1983;101:202-11. doi: 10.1016/0076-6879(83)01015-0.
9
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
10
Killer toxin from Hansenula mrakii selectively inhibits cell wall synthesis in a sensitive yeast.粟酒裂殖酵母产生的杀伤毒素可选择性抑制敏感酵母的细胞壁合成。
FEBS Lett. 1986 Mar 3;197(1-2):50-4. doi: 10.1016/0014-5793(86)80296-4.
Zotero 插件