Risatti J B, Capman W C, Stahl D A
Illinois State Geological Survey, Microbial Geochemistry Laboratory, Champaign 61820.
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10173-7. doi: 10.1073/pnas.91.21.10173.
Traditional studies of microbial communities are incomplete because of the inability to identify and quantify all contributing populations. In the present study, we directly determine the abundance and distribution of sulfate-reducing bacterial populations in a microbial mat community by using hybridization probes complementary to the 16S-like rRNAs of major phylogenetic groups. Most of the major groups were found in this single community, distributed for the most part in nonoverlapping depth intervals of the mat. The reflection of the phylogenetic structure in the community structure suggests that those species making up the major phylogenetic groups perform specific interrelated metabolic functions in the community. Comparison of population profiles to previously observed rates of sulfate reduction suggests there are additional populations of sulfate-reducing bacteria both within the photooxic zone and deeper in the mat.
由于无法识别和量化所有相关菌群，传统的微生物群落研究并不完整。在本研究中，我们通过使用与主要系统发育群体的16S样rRNA互补的杂交探针，直接确定了微生物垫群落中硫酸盐还原细菌群体的丰度和分布。在这个单一群落中发现了大多数主要群体，它们大多分布在垫子不重叠的深度区间内。群落结构中系统发育结构的反映表明，构成主要系统发育群体的那些物种在群落中执行特定的相互关联的代谢功能。将种群分布与先前观察到的硫酸盐还原速率进行比较表明，在光氧区和垫子更深的区域内都存在额外的硫酸盐还原细菌种群。
