Harmsen H J, Wullings B, Akkermans A D, Ludwig W, Stams A J
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
Arch Microbiol. 1993;160(3):238-40. doi: 10.1007/BF00249130.
A 16S rRNA sequence analysis of Syntrophobacter wolinii was done by using PCR amplification of the 16S rRNA-genes from DNA isolated from the S. wolinii-Desulfovibrio sp. coculture. Phylogenetic analysis using the obtained sequence revealed that S. wolinii was not related to bacteria growing syntrophically on other fatty acids than propionate, but was related to sulfate-reducing bacteria. The closest related bacteria are Desulfomonile tiedjei and Desulfoarculus baarsii.
通过对沃氏互营杆菌(Syntrophobacter wolinii)-脱硫弧菌属(Desulfovibrio sp.)共培养物中分离出的DNA进行16S rRNA基因的PCR扩增,对沃氏互营杆菌进行了16S rRNA序列分析。使用获得的序列进行系统发育分析表明,沃氏互营杆菌与利用除丙酸盐以外的其他脂肪酸进行互营生长的细菌无关,但与硫酸盐还原菌有关。亲缘关系最近的细菌是蒂氏脱硫单胞菌(Desulfomonile tiedjei)和巴斯氏脱硫弧菌(Desulfoarculus baarsii)。