Kondo M, Mitani H, Shima A
Zoological Institute, School of Medicine, Tokyo University, Japan.
Photochem Photobiol. 1994 Aug;60(2):120-4. doi: 10.1111/j.1751-1097.1994.tb05077.x.
Goldfish cells (RBCF-1) cultured at different cell densities were harvested and their photorepair (PR) abilities were examined in terms of survival. Photorepair ability gradually increased during the phase of logarithmic growth, reaching a maximum in cells at the confluent state. This enhancement of PR ability disappeared 12 h after replating of cells in fresh medium. A number of growth-inhibiting treatments (serum depletion, UVC, hydroxyurea [HU], change in incubation temperature) were tested for their ability to induce PR. The treatment of cells with HU and serum depletion induced PR while the other treatments did not. The increase in the ability to perform PR after treatment with HU or serum depletion returned to normal levels more rapidly than that after fluorescent light treatment.
收获在不同细胞密度下培养的金鱼细胞（RBCF-1），并根据存活率检测其光修复（PR）能力。光修复能力在对数生长期逐渐增强，在汇合状态的细胞中达到最大值。细胞重新接种到新鲜培养基中12小时后，这种PR能力的增强消失。测试了多种生长抑制处理（血清饥饿、紫外线C、羟基脲[HU]、孵育温度变化）诱导PR的能力。用HU和血清饥饿处理细胞可诱导PR，而其他处理则不能。与荧光处理后相比，用HU或血清饥饿处理后进行PR的能力恢复到正常水平的速度更快。
