Ugalde E, Corsi-Cabrera M, Juárez J, Ramos J, Arce C
Departamento de Psicofisiología, Facultad de Psicología, Universidad Nacional Autónoma de México.
Sleep. 1994 Apr;17(3):226-30. doi: 10.1093/sleep/17.3.226.
Effects of 6 hours of total sleep deprivation (TSD) by gentle handling and 6 hours of sleep on the waking electroencephalogram (EEG) activity of 14 Wistar rats were studied during the lights-on portion of the light-dark cycle under two TSD schedules: a) TSD 0800-1400 hours and sleep 1400-2000 hours, and b) sleep 0800-1400 hours and TSD 1400-2000 hours. EEG was recorded monopolarly from left and right parietals referred to ipsilateral reference electrodes. Spectral analysis was performed on samples of waking EEG during TSD (each 30 minutes) and during sleep (each 60 minutes after enforced awakening). The following significant changes were observed: TSD induced a linear increase in the absolute power of delta (1.46-3.42 Hz) and the full band (1.6-24.9 Hz) and produced a decrease in the interparietal correlation of theta. Sleep induced the opposite results. Theta relative power (power in a band expressed as a percentage of total power between 1.46 and 24.9 Hz) showed a circadian effect. It was higher at 1400 hours than at 0800 and 2000 hours after both sleep and TSD conditions. Six hours of TSD were enough to induce significant changes in the waking EEG regardless of position of TSD in the lights-on period of the light-dark cycle.
通过轻柔处理使14只Wistar大鼠完全睡眠剥夺（TSD）6小时，并给予6小时睡眠，在明暗周期的光照时段，按照两种TSD时间表研究其清醒脑电图（EEG）活动：a）TSD时间为08:00 - 14:00，睡眠时间为14:00 - 20:00；b）睡眠时间为08:00 - 14:00，TSD时间为14:00 - 20:00。EEG从左右顶叶单极记录，参考同侧参考电极。对TSD期间（每30分钟）和睡眠期间（强制唤醒后每60分钟）的清醒EEG样本进行频谱分析。观察到以下显著变化：TSD导致δ波（1.46 - 3.42 Hz）和全频段（1.6 - 24.9 Hz）的绝对功率呈线性增加，并使顶叶间θ波相关性降低。睡眠则产生相反的结果。θ波相对功率（某频段功率占1.46至24.9 Hz总功率的百分比）显示出昼夜节律效应。在睡眠和TSD条件下，14:00时的θ波相对功率均高于08:00和20:00时。无论TSD在明暗周期光照时段中的位置如何，6小时的TSD足以引起清醒EEG的显著变化。
