Piñeyro-López A, Martínez de Villarreal L, González-Alanís R
Departamento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.
Toxicology. 1994 Sep 6;92(1-3):217-27. doi: 10.1016/0300-483x(94)90179-1.
Toxin T-514 is a dimeric anthracenone isolated from the Karwinskia humboldtiana (buckthorn) plant. Its potential anti-neoplastic effect was evaluated in vitro and the results obtained were compared with the effect of other known anti-cancer agents. Normal and malignant continuous cell lines were tested. After a 72-h exposure, neoplastic cells derived from hepatic, pulmonary and colonic tissues were more sensitive to toxin T-514 than normal cells from the corresponding organ. Hepatoma cells and colon adenocarcinoma CT50 values were < 10 micrograms/ml. Lung adenocarcinoma, undifferentiated bronchogenic cancer cells and small cell carcinoma CT50 values were < 20 micrograms/ml. All benign cell CT50 levels tested were > 113 micrograms/ml. This in vitro selective toxicity found with toxin T-514 was also seen with 5-fluororacil and mitomycin for colon adenocarcinoma and with epidoxorubicin for undifferentiated bronchogenic cancer cells.
毒素T - 514是从洪堡氏卡氏鼠李（鼠李科鼠李属）植物中分离出的一种二聚蒽醌。对其潜在的抗肿瘤作用进行了体外评估，并将所得结果与其他已知抗癌药物的作用进行了比较。对正常和恶性连续细胞系进行了测试。暴露72小时后，源自肝、肺和结肠组织的肿瘤细胞比对相应器官的正常细胞对毒素T - 514更敏感。肝癌细胞和结肠腺癌的半数致死浓度（CT50）值<10微克/毫升。肺腺癌、未分化支气管癌细胞和小细胞癌的CT50值<20微克/毫升。所有测试的良性细胞的CT50水平均>113微克/毫升。毒素T - 514在体外发现的这种选择性毒性在5 - 氟尿嘧啶和丝裂霉素对结肠腺癌的作用以及表柔比星对未分化支气管癌细胞的作用中也有体现。
