卡氏南美茶毒素T-514及其非对映异构体在原代肝细胞培养物中的肝毒性比较。

Comparison of the hepatotoxicity of toxin T-514 of Karwinskia humboldtiana and its diastereoisomer in primary liver cell cultures.

作者信息

Garza-Ocañas L, Jiang T, Acosta D, Torres-Alanis O, Waksman de Torres N, Piñeyro-Lopez A

机构信息

Departmento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.

出版信息

Toxicon. 1994 Oct;32(10):1287-91. doi: 10.1016/0041-0101(94)90361-1.

DOI:10.1016/0041-0101(94)90361-1

PMID:7846701

Abstract

Toxin T-514 of Karwinskia humboldtiana has been demonstrated to be hepatotoxic in vivo and in vitro. Recently a diastereoisomer of T-514 has been isolated. In the present study we have evaluated and compared the in vitro hepatoxicity of the diastereoisomer of T-514 using primary cultures of rat hepatocytes. Cytotoxicity was evaluated by release of cytoplasmic enzyme lactate dehydrogenase (LDH), and mitochondrial metabolic function (MTT reduction). The diastereoisomer was shown to be almost as hepatoxic in vitro as toxin T-514.

摘要

洪堡卡罗琳木的毒素T - 514已被证实在体内和体外均具有肝毒性。最近，T - 514的一种非对映异构体已被分离出来。在本研究中，我们使用大鼠原代肝细胞培养物评估并比较了T - 514非对映异构体的体外肝毒性。通过细胞质酶乳酸脱氢酶（LDH）的释放和线粒体代谢功能（MTT还原）来评估细胞毒性。结果表明，该非对映异构体在体外的肝毒性几乎与毒素T - 514相同。