Velazco M R, Montero R, Rojas E, Gonsebatt M E, Sordo M, Piñeyro A, Ostrosky-Wegman P
Departamento de Farmacología y Toxicología, Facultad de Medicina, UANL, Colonia del Valle, Nuevo León, México.
Anticancer Drugs. 1996 Aug;7(6):710-5. doi: 10.1097/00001813-199608000-00013.
Toxicity by Karwinskia humboldtiana, a Rhamnaceae plant, has been assessed in a number of studies. Four dimmeric anthracenones, named T-496, T-514, T-516 and T-544 for their molecular weight, have been isolated from this plant. T-514, in particular, has been shown to be toxic to liver and lung as well as to tumoral cell lines, preferentially to those from liver tumors. For this reason it has been suggested that the toxin could be used as an antineoplastic agent. The present study was performed to characterize the biological activity of T-514 as a potential cytostatic and genotoxic agent. Peripheral blood lymphocytes in culture were used as a test system, where chromosomal aberrations and sister chromatid exchanges were scored in order to evaluate genotoxicity, and mitotic index and cell proliferation kinetics were used as parameters for cytostatic and cytotoxic ability. Genotoxicity to lymphocytes was negative. However, proliferation was affected by the toxin, demonstrating a cytostatic activity independent of genotoxic damage.
鼠李科植物洪堡鼠李的毒性已在多项研究中得到评估。从该植物中分离出了四种二聚蒽酮，根据其分子量分别命名为T-496、T-514、T-516和T-544。特别是T-514，已被证明对肝脏、肺以及肿瘤细胞系有毒性，对肝癌细胞系尤为明显。因此，有人提出该毒素可作为一种抗肿瘤药物。本研究旨在表征T-514作为潜在的细胞生长抑制剂和基因毒性剂的生物活性。培养的外周血淋巴细胞用作测试系统，通过对染色体畸变和姐妹染色单体交换进行评分来评估基因毒性，并将有丝分裂指数和细胞增殖动力学用作细胞生长抑制和细胞毒性能力的参数。对淋巴细胞的基因毒性为阴性。然而，该毒素影响了细胞增殖，表明其具有独立于基因毒性损伤的细胞生长抑制活性。
