Lalo D, Stettler S, Mariotte S, Gendreau E, Thuriaux P
Département der Biologie Cellulaire et Moléculaire, Gif sur Yvette, France.
Yeast. 1994 Apr;10(4):523-33. doi: 10.1002/yea.320100412.
A 15.1 kb fragment of the yeast genome was allocated to the centromeric region of chromosome XIV by genetic mapping. It contained six bona fide genes, RPC34, FUN34, CIT1 (Suissa et al., 1984), RLP7, PET8 and MRP7 (Fearon and Mason, 1988) and two large open reading frames, DOM34 and TOM34. RPC34 and RLP7 define strictly essential functions, whereas CIT1, PET8 and MRP7 encode mitochondrial proteins. The PET8 product belongs to a family of mitochondrial carrier proteins. FUN34 encodes a putative transmembraneous protein that is non-essential as judged from the normal growth of the fun34-::LUK18(URA3) allele even on respirable substrates. TOM34 codes for a putative RNA binding protein, and DOM34 defines a hypothetical polypeptide of 35 kDa, with no significant homology to known proteins. The region under study also contains two divergently transcribed tDNAs, separated only by a chimeric transposable element. This tight tDNA linkage pattern is commonly encountered in yeast, and a general hypothesis is proposed for its emergence on the Saccharomyces cerevisiae genome. RPC34, RLP7, PET8 and MRP7 are unique on the yeast genome, but the remaining genes belong to an extant centromeric duplication between chromosome III and XIV.
通过遗传图谱分析，将酵母基因组的一个15.1 kb片段定位到了第十四条染色体的着丝粒区域。它包含六个真正的基因，即RPC34、FUN34、CIT1（苏伊萨等人，1984年）、RLP7、PET8和MRP7（费伦和梅森，1988年），以及两个大的开放阅读框，DOM34和TOM34。RPC34和RLP7定义了绝对必需的功能，而CIT1、PET8和MRP7编码线粒体蛋白。PET8产物属于线粒体载体蛋白家族。FUN34编码一种假定的跨膜蛋白，从fun34-::LUK18(URA3)等位基因在可呼吸底物上的正常生长情况判断，该蛋白并非必需。TOM34编码一种假定的RNA结合蛋白，DOM34定义了一种35 kDa的假想多肽，与已知蛋白无明显同源性。所研究的区域还包含两个反向转录的tDNA，仅由一个嵌合转座元件隔开。这种紧密的tDNA连锁模式在酵母中很常见，并提出了一个关于其在酿酒酵母基因组上出现的一般假说。RPC34、RLP7、PET8和MRP7在酵母基因组上是独特的，但其余基因属于第三条染色体和第十四条染色体之间现存的着丝粒重复序列。
