Verhasselt P, Aert R, Voet M, Volckaert G
University of Leuven, Laboratory of Gene Technology, Belgium.
Yeast. 1994 Oct;10(10):1355-61. doi: 10.1002/yea.320101013.
The nucleotide sequence of 23.6 kb of the right arm of chromosome XIV is described, starting from the centromeric region. Both strands were sequenced with an average redundancy of 4.87 per base pair. The overall G+C content is 38.8% (42.5% for putative coding regions versus 29.4% for non-coding regions). Twelve open reading frames (ORFs) greater than 100 amino acids were detected. Codon frequencies of the twelve ORFs agree with codon usage in Saccharomyces cerevisiae and all show the characteristics of low level expressed genes. Five ORFs (N2019, N2029, N2031, N2048 and N2050) are encoded by previously sequenced genes (the mitochondrial citrate synthase gene, FUN34, RPC34, PRP2 and URK1, respectively). ORF N2052 shows the characteristics of a transmembrane protein. Other elements in this region are a tRNA(Pro) gene, a tRNA(Asn) gene, a tau 34 and a truncated delta 34 element. Nucleotide sequence comparison results in relocation of the SIS1 gene to the left arm of the chromosome as confirmed by colinearity analysis.
本文描述了从着丝粒区域开始的十四号染色体右臂23.6 kb的核苷酸序列。两条链均进行了测序，平均每碱基对的冗余度为4.87。总体G+C含量为38.8%（推定编码区为42.5%，非编码区为29.4%）。检测到12个大于100个氨基酸的开放阅读框（ORF）。这12个ORF的密码子频率与酿酒酵母中的密码子使用情况一致，均显示出低水平表达基因的特征。5个ORF（分别为N2019、N2029、N2031、N2048和N2050）由先前测序的基因编码（分别为线粒体柠檬酸合酶基因、FUN34、RPC34、PRP2和URK1）。ORF N2052显示出跨膜蛋白的特征。该区域的其他元件包括一个tRNA(Pro)基因、一个tRNA(Asn)基因、一个tau 34和一个截短的delta 34元件。核苷酸序列比较结果表明，SIS1基因已重新定位到染色体的左臂，共线性分析证实了这一点。
