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对酵母第十一染色体上一段24.6 kb的片段进行的全序列分析,确定了已知的URA1、SAC1和TRP3基因座,并揭示了6个新的开放阅读框,其中包括与苏氨酸脱水酶、膜转运蛋白、海因酶以及磷脂酶A2激活蛋白的同源物。

The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein.

作者信息

Tzermia M, Horaitis O, Alexandraki D

机构信息

Foundation for Research and Technology-HELLAS, Institute of Molecular Biology and Biotechnology, Crete, Greece.

出版信息

Yeast. 1994 May;10(5):663-79. doi: 10.1002/yea.320100511.

Abstract

We report the entire sequence of a 26.4 kb segment of chromosome XI of Saccharomyces cerevisiae. Identification of the known loci URA1, TRP3 and SAC1 revealed a translocation compared to the genetic map. Additionally, six unknown open reading frames have been identified. One of them is similar to catabolic threonine dehydratases. Another one contains characteristic features of membrane transporters. A third one is homologous in half of its length to the prokaryotic hydantoinase HyuA and in the other half to hydatoinase HyuB. A fourth one is homologous to the mammalian phospholipase A2-activating protein. A fifth one, finally, is homologous to the hypothetical open reading frame YCR007C of chromosome III. The sequence has been deposited in the EMBL data library under Accession Number X75951.

摘要

我们报道了酿酒酵母(Saccharomyces cerevisiae)第XI号染色体上一段26.4 kb片段的完整序列。已知基因座URA1、TRP3和SAC1的鉴定结果显示,与遗传图谱相比存在易位现象。此外,还鉴定出六个未知的开放阅读框。其中一个与分解代谢型苏氨酸脱水酶相似。另一个具有膜转运蛋白的特征。第三个在其长度的一半与原核海因酶HyuA同源,另一半与海因酶HyuB同源。第四个与哺乳动物磷脂酶A2激活蛋白同源。最后,第五个与第III号染色体上的假设开放阅读框YCR007C同源。该序列已存入EMBL数据库,登录号为X75951。

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