Rasmussen S W
Department of Physiology, Carlsberg Laboratory, Copenhagen, Denmark.
Yeast. 1994 Apr;10 Suppl A:S63-8. doi: 10.1002/yea.320100008.
The complete DNA sequence of cosmid clone pUKG148 comprising 28,600 base pairs was determined from an ordered set of subclones. The sequence contains 22 open reading frames longer than 100 amino acids of which five are entirely covered by other, longer reading frames. YKL054 exhibits 25% homology at the amino acid level to a number of plant storage proteins of the glutenin type, YKL056 is 40% homologous to a translationally controlled mammalian tumour protein, YKL058 (TOA2) is identical to the small subunit of transcription factor TFIIA from yeast and YKL060 is identical to the FBA1 gene also from yeast, already sequenced but not mapped to chromosome XI. The remaining 13 open reading frames show weak or no homology to known genes.
从一组有序的亚克隆中确定了包含28,600个碱基对的黏粒克隆pUKG148的完整DNA序列。该序列包含22个长度超过100个氨基酸的开放阅读框,其中5个完全被其他更长的阅读框覆盖。YKL054在氨基酸水平上与多种麦谷蛋白类型的植物贮藏蛋白具有25%的同源性,YKL056与一种翻译控制的哺乳动物肿瘤蛋白具有40%的同源性,YKL058(TOA2)与酵母转录因子TFIIA的小亚基相同,YKL060与同样来自酵母的FBA1基因相同,该基因已测序但未定位到XI号染色体上。其余13个开放阅读框与已知基因的同源性较弱或无同源性。