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酵母第十一染色体上一个28.6 kb区域的序列包括FBA1和TOA2基因、一个与翻译控制肿瘤蛋白相似的开放阅读框(ORF)、一个含有在植物贮藏蛋白中也能找到的基序的ORF以及13个与已知蛋白同源性较弱或无同源性的ORF。

Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins.

作者信息

Rasmussen S W

机构信息

Department of Physiology, Carlsberg Laboratory, Copenhagen, Denmark.

出版信息

Yeast. 1994 Apr;10 Suppl A:S63-8. doi: 10.1002/yea.320100008.

Abstract

The complete DNA sequence of cosmid clone pUKG148 comprising 28,600 base pairs was determined from an ordered set of subclones. The sequence contains 22 open reading frames longer than 100 amino acids of which five are entirely covered by other, longer reading frames. YKL054 exhibits 25% homology at the amino acid level to a number of plant storage proteins of the glutenin type, YKL056 is 40% homologous to a translationally controlled mammalian tumour protein, YKL058 (TOA2) is identical to the small subunit of transcription factor TFIIA from yeast and YKL060 is identical to the FBA1 gene also from yeast, already sequenced but not mapped to chromosome XI. The remaining 13 open reading frames show weak or no homology to known genes.

摘要

从一组有序的亚克隆中确定了包含28,600个碱基对的黏粒克隆pUKG148的完整DNA序列。该序列包含22个长度超过100个氨基酸的开放阅读框,其中5个完全被其他更长的阅读框覆盖。YKL054在氨基酸水平上与多种麦谷蛋白类型的植物贮藏蛋白具有25%的同源性,YKL056与一种翻译控制的哺乳动物肿瘤蛋白具有40%的同源性,YKL058(TOA2)与酵母转录因子TFIIA的小亚基相同,YKL060与同样来自酵母的FBA1基因相同,该基因已测序但未定位到XI号染色体上。其余13个开放阅读框与已知基因的同源性较弱或无同源性。

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