Leu A J, Berk D A, Yuan F, Jain R K
Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Boston 02114.
Am J Physiol. 1994 Oct;267(4 Pt 2):H1507-13. doi: 10.1152/ajpheart.1994.267.4.H1507.
The present study had two goals: 1) to establish an animal model in which a large network of the initial lymphatics of the skin can be investigated in vivo and 2) to measure effective flow velocity (defined as axial component of the flow velocity) in the lymph capillary network of the skin for the first time. A fluorescence microlymphography technique was used to stain the lymph capillaries in the superficial layer of the skin of the nude mouse tail in 10 female animals (mean age 45.8 +/- 2.4 days; mean wt 21.2 +/- 0.8 g). With the use of densitometric image analysis, effective flow velocity along the tail was measured. The network consisted of a honeycomb-like layer of hexagonally shaped meshes that could be stained in all animals. Effective lymph flow velocities were in the range of 1.4-20.4 microns/s with a mean value of 7.7 +/- 5.9 microns/s; median value was 6.2 microns/s (4.5-10.5; 25 and 75% percentiles). This new animal model allows studies of a large network of lymph capillaries in the skin and should provide new insight into the physiology and pathophysiology of the initial lymphatics.
1）建立一种动物模型，以便在体内研究皮肤初始淋巴管的大型网络；2）首次测量皮肤淋巴毛细管网络中的有效流速（定义为流速的轴向分量）。采用荧光显微淋巴管造影技术对10只雌性动物（平均年龄45.8±2.4天；平均体重21.2±0.8克）裸鼠尾巴皮肤表层的淋巴毛细管进行染色。使用密度图像分析方法测量沿尾巴的有效流速。该网络由六边形网格组成的蜂窝状层构成，所有动物的该层均可被染色。有效淋巴流速范围为1.4 - 20.4微米/秒，平均值为7.7±5.9微米/秒；中位数为6.2微米/秒（4.5 - 10.5；第25和75百分位数）。这种新的动物模型能够用于研究皮肤中大型淋巴毛细管网络，有望为初始淋巴管的生理学和病理生理学提供新的见解。
