Burek K A, Rossi C A, Leduc J W, Yuill T M
Department of Veterinary Science, University of Wisconsin, Madison.
Am J Trop Med Hyg. 1994 Sep;51(3):286-94. doi: 10.4269/ajtmh.1994.51.286.
The purposes of this study were to determine if hantaviruses were present in the Great Lakes port areas of Wisconsin and Minnesota and if so, to identify which virus and which rodent host species were involved. Rodents were trapped in Duluth, Minnesota, Superior, Green Bay, and Milwaukee, Wisconsin, all ports of call for international maritime shipping. A total of 675 wild rodents were captured and tested, including 310 meadow voles (Microtus pennsylvanicus), 173 Norway rats (Rattus norvegicus), 179 Peromyscus spp., (including white footed mice [P. leucopus] and deer mice [P. maniculatus gracilis and P. maniculatus bairdii]), and 13 house mice (Mus musculus). Twenty percent of the rats, 17% of the meadow voles, 8% of the house mice, and 3% of the Peromyscus spp. had antibody to a hantavirus by immunofluorescent antibody assay (IFA). By the plaque-reduction neutralization test (PRNT), nine of 36 meadow voles, one of 4 P. leucopus, and one of 34 rats had hantavirus antibody, with the highest titers to Prospect Hill (PH) virus. All of the PRNT-seropositive individuals were from the twin cities of Superior and Duluth. Hantavirus antigen was detected in lung tissue by IFA in M. pennsylvanicus and Peromyscus spp., but not in rats. Two hantaviruses, designated SD-1 and SD-2, were isolated from M. pennsylvanicus captured in Duluth and found to be very similar to prototype PH virus by cross-IFA and cross-PRNT. Virus isolation attempts were unsuccessful from tissues of the Peromyscus spp. and the rats.
本研究的目的是确定威斯康星州和明尼苏达州的五大湖港口地区是否存在汉坦病毒，如果存在，则确定涉及哪种病毒以及哪种啮齿动物宿主物种。在明尼苏达州的德卢斯、威斯康星州的苏必利尔、格林湾和密尔沃基捕获了啮齿动物，这些都是国际海运的停靠港。总共捕获并检测了675只野生啮齿动物，包括310只草甸田鼠（草原田鼠）、173只褐家鼠、179只白足鼠属（包括白足鼠和鹿鼠）和13只家鼠。通过免疫荧光抗体试验（IFA），20%的褐家鼠、17%的草甸田鼠、8%的家鼠和3%的白足鼠属对汉坦病毒有抗体。通过空斑减少中和试验（PRNT），36只草甸田鼠中有9只、4只白足鼠中有1只、34只褐家鼠中有1只具有汉坦病毒抗体，对展望山（PH）病毒的滴度最高。所有PRNT血清阳性个体均来自苏必利尔和德卢斯这两个双子城。通过IFA在草原田鼠和白足鼠属的肺组织中检测到汉坦病毒抗原，但在褐家鼠中未检测到。从在德卢斯捕获的草原田鼠中分离出两种汉坦病毒，分别命名为SD - 1和SD - 2，通过交叉IFA和交叉PRNT发现它们与原型PH病毒非常相似。从白足鼠属和褐家鼠的组织中进行病毒分离的尝试未成功。
